Nanoparticles of Conjugated Methotrexate-Human Serum Albumin : Preparation and Cytotoxicity Evaluations
Joint Authors
Derakhshan, Mohammad Ali
Ahadi, Fatemeh
Ghahremani, Mohammad Hossein
Amini, Mohsen
Dinarvand, Rassoul
Mansoori, Pooria
Salman Nouri, Faranak
Taheri, Azade
Ostad, Seyed Nasser
Atyabi, Fatemeh
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-09-13
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Engineering Sciences and Information Technology
Chemistry
Civil Engineering
Abstract EN
Methotrexate-human serum albumin conjugates were developed by a simple carbodiimide reaction.
Methotrexate-human serum albumin conjugates were then crosslinked with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide HCl (EDC) to form nanoparticles.
The size of nanoparticles determined by laser light scattering and TEM was between 90–150 nm.
Nanoparticles were very stable at physiologic conditions (PBS pH 7.4, 37°C) and after incubation with serum.
The effect of amount of EDC used for crosslinking on the particle size and free amino groups of nanoparticles was examined.
The amount of crosslinker showed no significant effect on the size of nanoparticles but free amino groups of nanoparticles were decreased by increasing the crosslinker.
The physicochemical interactions between methotrexate and human serum albumin were investigated by differential scanning calorimetry (DSC).
Nanoparticles were more cytotoxic on T47D cells compared to free methotrexate.
Moreover, methotrexate-human serum albumin nanoparticles decreased the IC50 value of methotrexate on T47D cells in comparison with free methotrexate.
American Psychological Association (APA)
Taheri, Azade& Atyabi, Fatemeh& Salman Nouri, Faranak& Ahadi, Fatemeh& Derakhshan, Mohammad Ali& Amini, Mohsen…[et al.]. 2010. Nanoparticles of Conjugated Methotrexate-Human Serum Albumin : Preparation and Cytotoxicity Evaluations. Journal of Nanomaterials،Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-497223
Modern Language Association (MLA)
Taheri, Azade…[et al.]. Nanoparticles of Conjugated Methotrexate-Human Serum Albumin : Preparation and Cytotoxicity Evaluations. Journal of Nanomaterials No. 2011 (2011), pp.1-7.
https://search.emarefa.net/detail/BIM-497223
American Medical Association (AMA)
Taheri, Azade& Atyabi, Fatemeh& Salman Nouri, Faranak& Ahadi, Fatemeh& Derakhshan, Mohammad Ali& Amini, Mohsen…[et al.]. Nanoparticles of Conjugated Methotrexate-Human Serum Albumin : Preparation and Cytotoxicity Evaluations. Journal of Nanomaterials. 2010. Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-497223
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-497223