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A cDNA Cloning of a Novel Alpha-Class Tyrosinase of Pinctada fucata : Its Expression Analysis and Characterization of the Expressed Protein
Joint Authors
Takagi, Ryousuke
Miyashita, Tomoyuki
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-04-10
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Tyrosinase plays an important role in the formation of the shell matrix and melanin synthesis in mollusks shells.
A cDNA clone encoding a 47 kDa protein was isolated from the pearl oyster Pinctada fucata.
The cDNA was 1,957 base pairs long and encodes a 417 residue protein that has extensive sequence identity with tyrosinase (polyphenol oxidase: EC 1.14.18.1).
This tyrosinase-like protein, termed PfTy, contains an N-terminal signal sequence and the two copper-binding domain signatures (CuA and CuB), suggesting that PfTy belongs to the α-subclass of type-3 copper proteins.
Enzyme activity of PfTy was examined by a spectrophotometric method using the translation product derived from an S30 T7 high-yield protein expression system.
Tyrosinase activity was seen in this recombinant product.
RT-PCR analysis showed that PfTy mRNA was expressed in the mantle pallial, but not in the mantle edge.
Therefore, PfTy may participate in insoluble shell matrix formation of the nacreous layer.
PfTy expression was also observed in the foot, liver, and adductor muscle, suggesting that PfTy participates in the synthesis of melanins, which are effective scavengers of free radicals formed in multiple intracellular oxidative processes.
This is the first report of a novel α-class tyrosinase from the pearl oyster P.
fucata.
American Psychological Association (APA)
Takagi, Ryousuke& Miyashita, Tomoyuki. 2014. A cDNA Cloning of a Novel Alpha-Class Tyrosinase of Pinctada fucata : Its Expression Analysis and Characterization of the Expressed Protein. Enzyme Research،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-497429
Modern Language Association (MLA)
Takagi, Ryousuke& Miyashita, Tomoyuki. A cDNA Cloning of a Novel Alpha-Class Tyrosinase of Pinctada fucata : Its Expression Analysis and Characterization of the Expressed Protein. Enzyme Research No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-497429
American Medical Association (AMA)
Takagi, Ryousuke& Miyashita, Tomoyuki. A cDNA Cloning of a Novel Alpha-Class Tyrosinase of Pinctada fucata : Its Expression Analysis and Characterization of the Expressed Protein. Enzyme Research. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-497429
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-497429