Exhaustive Training Increases Uncoupling Protein 2 Expression and Decreases Bcl-2Bax Ratio in Rat Skeletal Muscle

Abstract EN

This work investigates the effects of oxidative stress due to exhaustive training on uncoupling protein 2 (UCP2) and Bcl-2/Bax in rat skeletal muscles.

A total of 18 Sprague-Dawley female rats were randomly divided into three groups: the control group (CON), the trained control group (TC), and the exhaustive trained group (ET).

Malondialdehyde (MDA), superoxide dismutase (SOD), xanthine oxidase (XOD), ATPase, UCP2, and Bcl-2/Bax ratio in red gastrocnemius muscles were measured.

Exhaustive training induced ROS increase in red gastrocnemius muscles, which led to a decrease in the cell antiapoptotic ability (Bcl-2/Bax ratio).

An increase in UCP2 expression can reduce ROS production and affect mitochondrial energy production.

Thus, oxidative stress plays a significant role in overtraining.