AP-2α Inhibits c-MYC Induced Oxidative Stress and Apoptosis in HaCaT Human Keratinocytes
Joint Authors
Yu, Lei
Domann, Frederick E.
Hitchler, Michael J.
Goswami, Prabhat C.
Sarsour, Ehab H.
Sun, Wenqing
Klingelhutz, Aloysius J.
Source
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-12-24
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
AP-2α and c-MYC are important transcription factors involved in multiple cellular processes.
They each display the paradoxical capacities to stimulate both cell proliferation and apoptosis under different conditions.
In the present study we found that over expression of c-MYC was associated with accumulation of reactive oxygen species (ROS) and apoptosis in human keratinocytes, both of which were significantly inhibited by co-expression of AP-2.
The effects of AP-2 on c-MYC were active at several levels.
First, AP-2 and c-MYC were confirmed to interact at the protein level as previously described.
In addition, forced expression of AP-2 significantly decreased steady state levels of c-MYC mRNA and protein.
These findings suggested that AP-2 may have a direct effect on the c-myc gene.
Chromatin immunoprecipitation assays demonstrated that AP-2 proteins bound to a cluster of AP-2 binding sites located within a 2 kb upstream regulatory region of c-myc These results suggest that the negative regulation of AP-2 on c-MYC activity was achieved through binding of AP-2 protein to the c-myc gene.
The effects of AP-2 on c-MYC induced ROS accumulation and apoptosis in epidermal keratinocytes are likely to play an important role in cell growth, differentiation and carcinogenesis of the skin.
American Psychological Association (APA)
Yu, Lei& Hitchler, Michael J.& Sun, Wenqing& Sarsour, Ehab H.& Goswami, Prabhat C.& Klingelhutz, Aloysius J.…[et al.]. 2009. AP-2α Inhibits c-MYC Induced Oxidative Stress and Apoptosis in HaCaT Human Keratinocytes. Journal of Oncology،Vol. 2009, no. 2009, pp.1-9.
https://search.emarefa.net/detail/BIM-497463
Modern Language Association (MLA)
Yu, Lei…[et al.]. AP-2α Inhibits c-MYC Induced Oxidative Stress and Apoptosis in HaCaT Human Keratinocytes. Journal of Oncology No. 2009 (2009), pp.1-9.
https://search.emarefa.net/detail/BIM-497463
American Medical Association (AMA)
Yu, Lei& Hitchler, Michael J.& Sun, Wenqing& Sarsour, Ehab H.& Goswami, Prabhat C.& Klingelhutz, Aloysius J.…[et al.]. AP-2α Inhibits c-MYC Induced Oxidative Stress and Apoptosis in HaCaT Human Keratinocytes. Journal of Oncology. 2009. Vol. 2009, no. 2009, pp.1-9.
https://search.emarefa.net/detail/BIM-497463
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-497463