Nanobiosensor for Detection and Quantification of DNA Sequences in Degraded Mixed Meats
Joint Authors
Mustafa, Shuhaimi
Yusop, M. H. M.
Latif, Mohammad Abdul
Hashim, U.
Khan, A. A.
Bari, M. F.
Ali, M. E.
Dhahi, Th. S.
Che Man, Y. B.
Hakim, M. A.
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-07-27
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Engineering Sciences and Information Technology
Chemistry
Civil Engineering
Abstract EN
A novel class of nanobiosensor was developed by integrating a 27-nucleotide AluI fragment of swine cytochrome b (cytb) gene to a 3-nm diameter citrate-tannate coated gold nanoparticle (GNP).
The biosensor detected 0.5% and 1% pork in raw and 2.5-h autoclaved pork-beef binary admixtures in a single step without any separation or washing.
The hybridization kinetics of the hybrid sensor was studied with synthetic and AluI digested real pork targets from moderate to extreme target concentrations and a sigmoidal relationship was found.
Using the kinetic curve, a convenient method for quantifying and counting target DNA copy number was developed.
The accuracy of the method was over 90% and 80% for raw and autoclaved pork-beef binary admixtures in the range of 5–100% pork adulteration.
The biosensor probe identified a target DNA sequence that was several-folds shorter than a typical PCR-template.
This offered the detection and quantitation of potential targets in highly processed or degraded samples where PCR amplification was not possible due to template crisis.
The assay was a viable alternative approach of qPCR for detecting, quantifying and counting copy number of shorter size DNA sequences to address a wide ranging biological problem in food industry, diagnostic laboratories and forensic medicine.
American Psychological Association (APA)
Ali, M. E.& Hashim, U.& Mustafa, Shuhaimi& Che Man, Y. B.& Yusop, M. H. M.& Khan, A. A.…[et al.]. 2011. Nanobiosensor for Detection and Quantification of DNA Sequences in Degraded Mixed Meats. Journal of Nanomaterials،Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-497479
Modern Language Association (MLA)
Ali, M. E.…[et al.]. Nanobiosensor for Detection and Quantification of DNA Sequences in Degraded Mixed Meats. Journal of Nanomaterials No. 2011 (2011), pp.1-11.
https://search.emarefa.net/detail/BIM-497479
American Medical Association (AMA)
Ali, M. E.& Hashim, U.& Mustafa, Shuhaimi& Che Man, Y. B.& Yusop, M. H. M.& Khan, A. A.…[et al.]. Nanobiosensor for Detection and Quantification of DNA Sequences in Degraded Mixed Meats. Journal of Nanomaterials. 2011. Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-497479
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-497479