Comparison of Ligation-Mediated PCR Methods in Differentiation of Mycobacterium tuberculosis Strains
Joint Authors
Sajduda, Anna
Wojtasik, Arkadiusz
Brzostek, Anna
Zaczek, Anna
Dziadek, Jaroslaw
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-4, 4 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-02-16
Country of Publication
Egypt
No. of Pages
4
Main Subjects
Abstract EN
Fast and inexpensive identification of epidemiological links between limited number of Mycobacterium tuberculosis strains is required to initially evaluate hospital outbreaks, laboratory crosscontaminations, and family or small community transmissions.
The ligation-mediated PCR methods (LM-PCR) appear sufficiently discriminative and reproducible to be considered as a good candidate for such initial, epidemiological analysis.
Here, we compared the discriminative power of the recently developed in our laboratory fast ligation amplification polymorphism (FLAP) method with fast ligation-mediated PCR (FLiP).
Verification of the results was based on analyzing a set of reference strains and RFLP-IS6110 typing.
The HGDI value was very similar for both LM-PCR methods and RFLP-IS6110 typing.
However, only 52% of strains were correspondingly grouped by both FLiP and FLAP methods.
Differentiation by FLAP method demonstrated a limited similarity to IS6110-RFLP (37,7%).
As much as 78,7% of strains were grouped identically when differentiated by FLiP and IS6110-RFLP methods.
The analysis differentiated 31, 35, and 36 groups when using FLAP, FLiP, and RFLP-IS6110 methods, respectively.
American Psychological Association (APA)
Zaczek, Anna& Brzostek, Anna& Wojtasik, Arkadiusz& Sajduda, Anna& Dziadek, Jaroslaw. 2014. Comparison of Ligation-Mediated PCR Methods in Differentiation of Mycobacterium tuberculosis Strains. BioMed Research International،Vol. 2014, no. 2014, pp.1-4.
https://search.emarefa.net/detail/BIM-497585
Modern Language Association (MLA)
Zaczek, Anna…[et al.]. Comparison of Ligation-Mediated PCR Methods in Differentiation of Mycobacterium tuberculosis Strains. BioMed Research International No. 2014 (2014), pp.1-4.
https://search.emarefa.net/detail/BIM-497585
American Medical Association (AMA)
Zaczek, Anna& Brzostek, Anna& Wojtasik, Arkadiusz& Sajduda, Anna& Dziadek, Jaroslaw. Comparison of Ligation-Mediated PCR Methods in Differentiation of Mycobacterium tuberculosis Strains. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-4.
https://search.emarefa.net/detail/BIM-497585
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-497585