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Preconditioning and Acute Effects of Flavonoids in Protecting Cardiomyocytes from Oxidative Cell Death
Joint Authors
Bandy, Brian
Akhlaghi, Masoumeh
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-07-05
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
While flavonoids can reportedly protect against cardiac ischemia-reperfusion injury, the relative effectiveness of different flavonoids and the mechanisms involved are unclear.
We compared protection by different flavonoids using rat embryonic ventricular H9c2 cells subjected to simulated ischemia-reperfusion (IR) and to tert-butyl hydroperoxide (t-buOOH).
Characterization of the IR model showed the relative contributions of glucose, serum, and oxygen deprivation to cell death.
With long-term (2-3 day) pretreatment before IR the best protection was given by catechin, epigallocatechin gallate, proanthocyanidins, and ascorbate, which protected at all doses.
Quercetin protected (34%) at 5 μM but was cytotoxic at higher doses.
Cyanidin protected mildly (10–15%) at 5 and 20 μM, while delphinidin had no effect at 5 μM and was cytotoxic at higher doses.
Comparing long-term and acute protection by catechin, a higher concentration was needed for benefit with acute (1 hr) pretreatment.
With a pure oxidative stress (t-buOOH) only quercetin significantly protected with 3-day pretreatment, while with short-term (1 h) pretreatments protection was best with quercetin and epigallocatechin gallate.
The results suggest catechins to be especially useful as IR preconditioning agents, while quercetin and epigallocatechin gallate may be the most protective acutely in situations of oxidative stress.
American Psychological Association (APA)
Akhlaghi, Masoumeh& Bandy, Brian. 2012. Preconditioning and Acute Effects of Flavonoids in Protecting Cardiomyocytes from Oxidative Cell Death. Oxidative Medicine and Cellular Longevity،Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-497600
Modern Language Association (MLA)
Akhlaghi, Masoumeh& Bandy, Brian. Preconditioning and Acute Effects of Flavonoids in Protecting Cardiomyocytes from Oxidative Cell Death. Oxidative Medicine and Cellular Longevity No. 2012 (2012), pp.1-9.
https://search.emarefa.net/detail/BIM-497600
American Medical Association (AMA)
Akhlaghi, Masoumeh& Bandy, Brian. Preconditioning and Acute Effects of Flavonoids in Protecting Cardiomyocytes from Oxidative Cell Death. Oxidative Medicine and Cellular Longevity. 2012. Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-497600
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-497600