Gemi : PCR Primers Prediction from Multiple Alignments

Abstract EN

Designing primers and probes for polymerase chain reaction (PCR) is a preliminary and critical step that requires the identification of highly conserved regions in a given set of sequences.

This task can be challenging if the targeted sequences display a high level of diversity, as frequently encountered in microbiologic studies.

We developed Gemi, an automated, fast, and easy-to-use bioinformatics tool with a user-friendly interface to design primers and probes based on multiple aligned sequences.

This tool can be used for the purpose of real-time and conventional PCR and can deal efficiently with large sets of sequences of a large size.