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Architecture of Y-Family DNA Polymerases Relevant to Translesion DNA Synthesis as Revealed in Structural and Molecular Modeling Studies
Joint Authors
Jacobs, Christopher
Loechler, Edward L.
Chandani, Sushil
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-20, 20 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-09-16
Country of Publication
Egypt
No. of Pages
20
Main Subjects
Abstract EN
DNA adducts, which block replicative DNA polymerases (DNAPs), are often bypassed by lesion-bypass DNAPs, which are mostly in the Y-Family.
Y-Family DNAPs can do non-mutagenic or mutagenic dNTP insertion, and understanding this difference is important, because mutations transform normal into tumorigenic cells.
Y-Family DNAP architecture that dictates mechanism, as revealed in structural and modeling studies, is considered.
Steps from adduct blockage of replicative DNAPs, to bypass by a lesion-bypass DNAP, to resumption of synthesis by a replicative DNAP are described.
Catalytic steps and protein conformational changes are considered.
One adduct is analyzed in greater detail: the major benzo[a]pyrene adduct (B[a]P-N2-dG), which is bypassed non-mutagenically (dCTP insertion) by Y-family DNAPs in the IV/κ-class and mutagenically (dATP insertion) by V/η-class Y-Family DNAPs.
Important architectural differences between IV/κ-class versus V/η-class DNAPs are discussed, including insights gained by analyzing ~400 sequences each for bacterial DNAPs IV and V, along with sequences from eukaryotic DNAPs kappa, eta and iota.
The little finger domains of Y-Family DNAPs do not show sequence conservation; however, their structures are remarkably similar due to the presence of a core of hydrophobic amino acids, whose exact identity is less important than the hydrophobic amino acid spacing.
American Psychological Association (APA)
Chandani, Sushil& Jacobs, Christopher& Loechler, Edward L.. 2010. Architecture of Y-Family DNA Polymerases Relevant to Translesion DNA Synthesis as Revealed in Structural and Molecular Modeling Studies. Journal of Nucleic Acids،Vol. 2010, no. 2010, pp.1-20.
https://search.emarefa.net/detail/BIM-497793
Modern Language Association (MLA)
Chandani, Sushil…[et al.]. Architecture of Y-Family DNA Polymerases Relevant to Translesion DNA Synthesis as Revealed in Structural and Molecular Modeling Studies. Journal of Nucleic Acids No. 2010 (2010), pp.1-20.
https://search.emarefa.net/detail/BIM-497793
American Medical Association (AMA)
Chandani, Sushil& Jacobs, Christopher& Loechler, Edward L.. Architecture of Y-Family DNA Polymerases Relevant to Translesion DNA Synthesis as Revealed in Structural and Molecular Modeling Studies. Journal of Nucleic Acids. 2010. Vol. 2010, no. 2010, pp.1-20.
https://search.emarefa.net/detail/BIM-497793
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-497793