Analysis of the Kanamycin in Raw Milk Using the Suspension Array
Joint Authors
Li, Jinfeng
Zhu, Xiaoyan
Zou, Mingqiang
Wang, Yanfei
Han, Yi
Zhang, Fan
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-4, 4 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-09-03
Country of Publication
Egypt
No. of Pages
4
Main Subjects
Abstract EN
With the monoclonal antibody against kanamycin being prepared successfully, a bead-based indirect competitive fluorescent immunoassay was developed to detect kanamycin in milk.
The fact that there was no significant cross-reaction with other aminoglycoside antibiotics implied that the monoclonal antibody was highly specific for kanamycin.
The limit of detection (LOD) and the 50% inhibition concentration (IC50) in raw milk were 3.2 ng/mL and 52.5 ng/mL, respectively.
Using the method developed in this study, the kanamycin concentrations were monitored in raw milk after the intramuscular administration of kanamycin in sick cows.
Compared to the conventional enzyme-linked immunosorbent assay (ELISA), the method using the suspension array system was more sensitive.
The results obtained in the present study showed a good correlation with that of the ELISA.
American Psychological Association (APA)
Wang, Yanfei& Zou, Mingqiang& Han, Yi& Zhang, Fan& Li, Jinfeng& Zhu, Xiaoyan. 2012. Analysis of the Kanamycin in Raw Milk Using the Suspension Array. Journal of Chemistry،Vol. 2013, no. 2013, pp.1-4.
https://search.emarefa.net/detail/BIM-497833
Modern Language Association (MLA)
Wang, Yanfei…[et al.]. Analysis of the Kanamycin in Raw Milk Using the Suspension Array. Journal of Chemistry No. 2013 (2013), pp.1-4.
https://search.emarefa.net/detail/BIM-497833
American Medical Association (AMA)
Wang, Yanfei& Zou, Mingqiang& Han, Yi& Zhang, Fan& Li, Jinfeng& Zhu, Xiaoyan. Analysis of the Kanamycin in Raw Milk Using the Suspension Array. Journal of Chemistry. 2012. Vol. 2013, no. 2013, pp.1-4.
https://search.emarefa.net/detail/BIM-497833
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-497833