Analysis of the Kanamycin in Raw Milk Using the Suspension Array

Abstract EN

With the monoclonal antibody against kanamycin being prepared successfully, a bead-based indirect competitive fluorescent immunoassay was developed to detect kanamycin in milk.

The fact that there was no significant cross-reaction with other aminoglycoside antibiotics implied that the monoclonal antibody was highly specific for kanamycin.

The limit of detection (LOD) and the 50% inhibition concentration (IC50) in raw milk were 3.2 ng/mL and 52.5 ng/mL, respectively.

Using the method developed in this study, the kanamycin concentrations were monitored in raw milk after the intramuscular administration of kanamycin in sick cows.

Compared to the conventional enzyme-linked immunosorbent assay (ELISA), the method using the suspension array system was more sensitive.

The results obtained in the present study showed a good correlation with that of the ELISA.