Purification and Properties of White Muscle Lactate Dehydrogenase from the Anoxia-Tolerant Turtle, the Red-Eared Slider, Trachemys scripta elegans
Joint Authors
Dawson, Neal J.
Bell, Ryan A. V.
Storey, Kenneth B.
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-02-21
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Lactate dehydrogenase (LDH; E.C.
1.1.1.27) is a crucial enzyme involved in energy metabolism in muscle, facilitating the production of ATP via glycolysis during oxygen deprivation by recycling NAD+.
The present study investigated purified LDH from the muscle of 20 h anoxic and normoxic T.
s.
elegans, and LDH from anoxic muscle showed a significantly lower (47%) Km for L-lactate and a higher Vmax value than the normoxic form.
Several lines of evidence indicated that LDH was converted to a low phosphate form under anoxia: (a) stimulation of endogenously present protein phosphatases decreased the Km of L-lactate of control LDH to anoxic levels, whereas (b) stimulation of kinases increased the Km of L-lactate of anoxic LDH to normoxic levels, and (c) dot blot analysis shows significantly less serine (78%) and threonine (58%) phosphorylation in anoxic muscle LDH as compared to normoxic LDH.
The physiological consequence of anoxia-induced LDH dephosphorylation appears to be an increase in LDH activity to promote the reduction of pyruvate in muscle tissue, converting the glycolytic end product to lactate to maintain a prolonged glycolytic flux under energy-stressed anoxic conditions.
American Psychological Association (APA)
Dawson, Neal J.& Bell, Ryan A. V.& Storey, Kenneth B.. 2013. Purification and Properties of White Muscle Lactate Dehydrogenase from the Anoxia-Tolerant Turtle, the Red-Eared Slider, Trachemys scripta elegans. Enzyme Research،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-497873
Modern Language Association (MLA)
Dawson, Neal J.…[et al.]. Purification and Properties of White Muscle Lactate Dehydrogenase from the Anoxia-Tolerant Turtle, the Red-Eared Slider, Trachemys scripta elegans. Enzyme Research No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-497873
American Medical Association (AMA)
Dawson, Neal J.& Bell, Ryan A. V.& Storey, Kenneth B.. Purification and Properties of White Muscle Lactate Dehydrogenase from the Anoxia-Tolerant Turtle, the Red-Eared Slider, Trachemys scripta elegans. Enzyme Research. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-497873
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-497873