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The utility of direct agglutination (dat) and fast agglutination screening (fast) tests in serodiagnosis of experimental microsporidiosis
Joint Authors
Abu al-Naja, Iman F.
Jafar, Maha R.
al-Zawawi, Lubna A.
al-Sayyid, Doaa
Muslim, Sherin F.
Source
Journal of the Egyptian Society of Parasitology
Issue
Vol. 38, Issue 3 (31 Dec. 2008), pp.903-918, 16 p.
Publisher
The Egyptian Society of Parasitology
Publication Date
2008-12-31
Country of Publication
Egypt
No. of Pages
16
Main Subjects
Abstract EN
The present study was designed to evaluate the efficiency of two serodiagnostic tests ; the direct agglutination test (DAT) and the fast agglutination screening test (FAST) in the diagnosis of Microsporidia in experimentally infected mice and to differentiate between different species of the parasite.
The swiss albino mice were divided into non infected control and infected experimental groups which were further subdivided into ten subgroups.
Ten samples of microsporidial spores were isolated from ten human stools and each one was used to infect each subgroup of mice.
Stool and sera were collected weekly from each subgroup from the 1st to the 4th week post infection (PI).
DAT & FAST tests, using antigen prepared from the different species of microsporidial spores were used to detect antibodies in sera of different mice subgroups.
The cross reactivity of microsporidial spores with the antibodies of Cyclospora cyatenensis and Cryptosporidium parvum was investigated by DAT & FAST.
The results proved that DAT & FAST were effective in detecting microsporidial antibodies in sera of experimentally infected mice from the 2nd week PI till the end of the study, without cross reactivity with C.
cyatenensis or C.
parvum.
They failed to differentiate between different Microspoiridia species used but, they gave good interpretation and they were specific and sensitive, and did not need sophisticated equipments.
American Psychological Association (APA)
Abu al-Naja, Iman F.& Jafar, Maha R.& al-Zawawi, Lubna A.& al-Sayyid, Doaa& Muslim, Sherin F.. 2008. The utility of direct agglutination (dat) and fast agglutination screening (fast) tests in serodiagnosis of experimental microsporidiosis. Journal of the Egyptian Society of Parasitology،Vol. 38, no. 3, pp.903-918.
https://search.emarefa.net/detail/BIM-49793
Modern Language Association (MLA)
Abu al-Naja, Iman F.…[et al.]. The utility of direct agglutination (dat) and fast agglutination screening (fast) tests in serodiagnosis of experimental microsporidiosis. Journal of the Egyptian Society of Parasitology Vol. 38, no. 3 (Dec. 2008), pp.903-918.
https://search.emarefa.net/detail/BIM-49793
American Medical Association (AMA)
Abu al-Naja, Iman F.& Jafar, Maha R.& al-Zawawi, Lubna A.& al-Sayyid, Doaa& Muslim, Sherin F.. The utility of direct agglutination (dat) and fast agglutination screening (fast) tests in serodiagnosis of experimental microsporidiosis. Journal of the Egyptian Society of Parasitology. 2008. Vol. 38, no. 3, pp.903-918.
https://search.emarefa.net/detail/BIM-49793
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 914-918
Record ID
BIM-49793