Combined Stimulation with the Tumor Necrosis Factor α and the Epidermal Growth Factor Promotes the Proliferation of Hepatocytes in Rat Liver Cultured Slices
Joint Authors
Guguen-Guillouzo, Christiane
Bichet, Nicole
Desmots, Fabienne
Masson, Régis
Finot, Francis
Vericat, Joan A.
Lafouge, Patricia
Ribault, Catherine
Loyer, Pascal
Source
International Journal of Hepatology
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-10-16
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
The culture liver slices are mainly used to investigate drug metabolism and xenobiotic-mediated liver injuries while apoptosis and proliferation remain unexplored in this culture model.
Here, we show a transient increase in LDH release and caspase activities indicating an ischemic injury during the slicing procedure.
Then, caspase activities decrease and remain low in cultured slices demonstrating a low level of apoptosis.
The slicing procedure is also associated with the G0/G1 transition of hepatocytes demonstrated by the activation of stress and proliferation signalling pathways including the ERK1/2 and JNK1/2/3 MAPKinases and the transient upregulation of c-fos.
The cells further progress up to mid-G1 phase as indicated by the sequential induction of c-myc and p53 mRNA levels after the slicing procedure and at 24 h of culture, respectively.
The stimulation by epidermal growth factor induces the ERK1/2 phosphorylation but fails to activate expression of late G1 and S phase markers such as cyclin D1 and Cdk1 indicating that hepatocytes are arrested in mid-G1 phase of the cell cycle.
However, we found that combined stimulation by the proinflammatory cytokine tumor necrosis factor α and the epidermal growth factor promotes the commitment to DNA replication as observed in vivo during the liver regeneration.
American Psychological Association (APA)
Finot, Francis& Masson, Régis& Desmots, Fabienne& Ribault, Catherine& Bichet, Nicole& Vericat, Joan A.…[et al.]. 2012. Combined Stimulation with the Tumor Necrosis Factor α and the Epidermal Growth Factor Promotes the Proliferation of Hepatocytes in Rat Liver Cultured Slices. International Journal of Hepatology،Vol. 2012, no. 2012, pp.1-14.
https://search.emarefa.net/detail/BIM-497951
Modern Language Association (MLA)
Finot, Francis…[et al.]. Combined Stimulation with the Tumor Necrosis Factor α and the Epidermal Growth Factor Promotes the Proliferation of Hepatocytes in Rat Liver Cultured Slices. International Journal of Hepatology No. 2012 (2012), pp.1-14.
https://search.emarefa.net/detail/BIM-497951
American Medical Association (AMA)
Finot, Francis& Masson, Régis& Desmots, Fabienne& Ribault, Catherine& Bichet, Nicole& Vericat, Joan A.…[et al.]. Combined Stimulation with the Tumor Necrosis Factor α and the Epidermal Growth Factor Promotes the Proliferation of Hepatocytes in Rat Liver Cultured Slices. International Journal of Hepatology. 2012. Vol. 2012, no. 2012, pp.1-14.
https://search.emarefa.net/detail/BIM-497951
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-497951