![](/images/graphics-bg.png)
Analysis of KRAS Mutations of Exon 2 Codons 12 and 13 by SNaPshot Analysis in Comparison to Common DNA Sequencing
Joint Authors
Fisseler-Eckhoff, Annette
Zinsky, Rica
Schirren, Joachim
Bölükbas, Servet
Bartsch, Holger
Source
Gastroenterology Research and Practice
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-12-21
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Due to the call for fast KRAS mutation status analysis for treatment of patients with monoclonal antibodies for metastatic colorectal cancer, sensitive, economic, and easily feasible methods are required.
Under this aspect, the sensitivity and specificity of the SNaPshot analysis in comparison to the commonly used DNA sequencing was checked.
We examined KRAS mutations in exon 2 codons 12 and 13 with DNA sequencing and SNaPshot analysis in 100 formalin-fixed paraffin-embedded tumor tissue samples of pancreatic carcinoma, colorectal carcinoma, and nonsmall cell lung cancer specimens of the primary tumor or metastases.
40% of these samples demonstrated mutated KRAS genes using sequencing and SNaPshot-analysis; additional five samples (45/100) were identified only with the SNaPshot.
KRAS mutation detection is feasible with the reliable SNaPshot analysis method.
The more frequent mutation detection by the SNaPshot analysis shows that this method has a high probability of accuracy in the detection of KRAS mutations compared to sequencing.
American Psychological Association (APA)
Zinsky, Rica& Bölükbas, Servet& Bartsch, Holger& Schirren, Joachim& Fisseler-Eckhoff, Annette. 2010. Analysis of KRAS Mutations of Exon 2 Codons 12 and 13 by SNaPshot Analysis in Comparison to Common DNA Sequencing. Gastroenterology Research and Practice،Vol. 2010, no. 2010, pp.1-5.
https://search.emarefa.net/detail/BIM-498183
Modern Language Association (MLA)
Zinsky, Rica…[et al.]. Analysis of KRAS Mutations of Exon 2 Codons 12 and 13 by SNaPshot Analysis in Comparison to Common DNA Sequencing. Gastroenterology Research and Practice No. 2010 (2010), pp.1-5.
https://search.emarefa.net/detail/BIM-498183
American Medical Association (AMA)
Zinsky, Rica& Bölükbas, Servet& Bartsch, Holger& Schirren, Joachim& Fisseler-Eckhoff, Annette. Analysis of KRAS Mutations of Exon 2 Codons 12 and 13 by SNaPshot Analysis in Comparison to Common DNA Sequencing. Gastroenterology Research and Practice. 2010. Vol. 2010, no. 2010, pp.1-5.
https://search.emarefa.net/detail/BIM-498183
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-498183