Functional Differences between the N-Terminal Domains of Mouse and Human Myosin Binding Protein-C
Joint Authors
Bezold, Kristina L.
Wong, Peony
Harris, Samantha P.
Shaffer, Justin F.
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-04-07
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The N-terminus of cMyBP-C can activate actomyosin interactions in the absence of Ca2+, but it is unclear which domains are necessary.
Prior studies suggested that the Pro-Ala rich region of human cMyBP-C activated force in permeabilized human cardiomyocytes, whereas the C1 and M-domains of mouse cMyBP-C activated force in permeabilized rat cardiac trabeculae.
Because the amino acid sequence of the P/A region differs between human and mouse cMyBP-C isoforms (46% identity), we investigated whether species-specific differences in the P/A region could account for differences in activating effects.
Using chimeric fusion proteins containing combinations of human and mouse C0, Pro-Ala, and C1 domains, we demonstrate here that the human P/A and C1 domains activate actomyosin interactions, whereas the same regions of mouse cMyBP-C are less effective.
These results suggest that species-specific differences between homologous cMyBP-C isoforms confer differential effects that could fine-tune cMyBP-C function in hearts of different species.
American Psychological Association (APA)
Shaffer, Justin F.& Wong, Peony& Bezold, Kristina L.& Harris, Samantha P.. 2010. Functional Differences between the N-Terminal Domains of Mouse and Human Myosin Binding Protein-C. Journal of Biomedicine and Biotechnology،Vol. 2010, no. 2010, pp.1-9.
https://search.emarefa.net/detail/BIM-498245
Modern Language Association (MLA)
Shaffer, Justin F.…[et al.]. Functional Differences between the N-Terminal Domains of Mouse and Human Myosin Binding Protein-C. Journal of Biomedicine and Biotechnology No. 2010 (2010), pp.1-9.
https://search.emarefa.net/detail/BIM-498245
American Medical Association (AMA)
Shaffer, Justin F.& Wong, Peony& Bezold, Kristina L.& Harris, Samantha P.. Functional Differences between the N-Terminal Domains of Mouse and Human Myosin Binding Protein-C. Journal of Biomedicine and Biotechnology. 2010. Vol. 2010, no. 2010, pp.1-9.
https://search.emarefa.net/detail/BIM-498245
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-498245