High Prevalence of Panton-Valentine Leukocidin (PVL) Genes in Nosocomial-Acquired Staphylococcus aureus Isolated from Tertiary Care Hospitals in Nepal
Joint Authors
Raj, V. Samuel
Singh, Winny
Shrestha, Bidya
Mohapatra, Tribhuban Mohan
Pokhrel, Bharat Mani
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-06-17
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Methicillin-resistant Staphylococcus aureus (MRSA) carrying the important virulence determinant, Panton-Valentine leukocidin (PVL), is an emerging infectious pathogen associated with skin and soft tissue infections as well as life-threatening invasive diseases.
In carrying out the first PVL prevalence study in Nepal, we screened 73 nosocomial isolates of S.
aureus from 2 tertiary care Nepali hospitals and obtained an overall PVL-positivity rate of 35.6% among the hospital isolates: 26.1% of MRSA and 51.9% of methicillin sensitive S.
aureus (MSSA) isolates were found to be positive for the PVL genes.
PVL prevalence was not associated with a specific (i) infection site, (ii) age group, or (iii) hospital of origin.
It was found to be positively associated with heterogeneous MRSA (73.3%) compared to homogeneous MRSA (3.2%) and MSSA (51.9%); negatively associated with multiresistant MRSA (22%) compared to nonmultiresistant MRSA (60%) and MSSA (51.9%); and positively associated with macrolide-streptogramin B resistance (93.8%) compared to macrolide-lincosamide-streptogramin B resistance (0%) and no-resistance (45.8%) types.
Macrolide-streptogramin B resistance was confirmed by the presence of msr(A) gene.
Restriction pattern analyses provided evidence to support the circulation of a limited number of clones of PVL-positive MRSA, arguing for the adaptability of these isolates to a hospital setting.
American Psychological Association (APA)
Shrestha, Bidya& Singh, Winny& Raj, V. Samuel& Pokhrel, Bharat Mani& Mohapatra, Tribhuban Mohan. 2014. High Prevalence of Panton-Valentine Leukocidin (PVL) Genes in Nosocomial-Acquired Staphylococcus aureus Isolated from Tertiary Care Hospitals in Nepal. BioMed Research International،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-498283
Modern Language Association (MLA)
Shrestha, Bidya…[et al.]. High Prevalence of Panton-Valentine Leukocidin (PVL) Genes in Nosocomial-Acquired Staphylococcus aureus Isolated from Tertiary Care Hospitals in Nepal. BioMed Research International No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-498283
American Medical Association (AMA)
Shrestha, Bidya& Singh, Winny& Raj, V. Samuel& Pokhrel, Bharat Mani& Mohapatra, Tribhuban Mohan. High Prevalence of Panton-Valentine Leukocidin (PVL) Genes in Nosocomial-Acquired Staphylococcus aureus Isolated from Tertiary Care Hospitals in Nepal. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-498283
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-498283