Signal Peptidase Complex Subunit 1 and Hydroxyacyl-CoA Dehydrogenase Beta Subunit Are Suitable Reference Genes in Human Lungs
Joint Authors
Ling, Maurice H. T.
Too, Issac H. K.
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-12-28
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Lung cancer is a common cancer, and expression profiling can provide an accurate indication to advance the medical intervention.
However, this requires the availability of stably expressed genes as reference.
Recent studies had shown that genes that are stably expressed in a tissue may not be stably expressed in other tissues suggesting the need to identify stably expressed genes in each tissue for use as reference genes.
DNA microarray analysis has been used to identify those reference genes with low fluctuation.
Fourteen datasets with different lung conditions were employed in our study.
Coefficient of variance, followed by NormFinder, was used to identify stably expressed genes.
Our results showed that classical reference genes such as GAPDH and HPRT1 were highly variable; thus, they are unsuitable as reference genes.
Signal peptidase complex subunit 1 (SPCS1) and hydroxyacyl-CoA dehydrogenase beta subunit (HADHB), which are involved in fundamental biochemical processes, demonstrated high expression stability suggesting their suitability in human lung cell profiling.
American Psychological Association (APA)
Too, Issac H. K.& Ling, Maurice H. T.. 2011. Signal Peptidase Complex Subunit 1 and Hydroxyacyl-CoA Dehydrogenase Beta Subunit Are Suitable Reference Genes in Human Lungs. ISRN Bioinformatics،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-498289
Modern Language Association (MLA)
Too, Issac H. K.& Ling, Maurice H. T.. Signal Peptidase Complex Subunit 1 and Hydroxyacyl-CoA Dehydrogenase Beta Subunit Are Suitable Reference Genes in Human Lungs. ISRN Bioinformatics No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-498289
American Medical Association (AMA)
Too, Issac H. K.& Ling, Maurice H. T.. Signal Peptidase Complex Subunit 1 and Hydroxyacyl-CoA Dehydrogenase Beta Subunit Are Suitable Reference Genes in Human Lungs. ISRN Bioinformatics. 2011. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-498289
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-498289