LMW Heparin Prevents Increased Kidney Expression of Proinflammatory Mediators in (NZBxNZW)F1 Mice
Joint Authors
Rekvig, Ole Petter
Hedberg, Annica
Fenton, Kristin Andreassen
Kanapathippillai, Premasany
Source
Clinical and Developmental Immunology
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-09-17
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
We have previously demonstrated that continuous infusion of low molecular weight (LMW) heparin delays autoantibody production and development of lupus nephritis in (NZBxNZW)F1 (B/W) mice.
In this study we investigated the effect of LMW heparin on renal cytokine and chemokine expression and on nucleosome-mediated activation of nucleosome-specific splenocytes.
Total mRNA extracted from kidneys of heparin-treated or -untreated B/W mice was analysed by qPCR for the expression of several cytokines, chemokines, and Toll-like receptors.
Splenocytes taken from B/W mice were stimulated with nucleosomes with or without the presence of heparin.
Splenocyte cell proliferation as thymidine incorporation and the expression of costimulatory molecules and cell activation markers were measured.
Heparin treatment of B/W mice reduced the in vivo expression of CCR2, IL1β, and TLR7 compared to untreated B/W mice.
Nucleosome-induced cell proliferation of splenocytes was not influenced by heparin.
The expression of CD80, CD86, CD69, CD25, CTLA-4, and TLR 2, 7, 8, and 9 was upregulated upon stimulation by nucleosomes, irrespective of whether heparin was added to the cell culture or not.
In conclusion, treatment with heparin lowers the kidney expression of proinflammatory mediators in B/W mice but does not affect nucleosomal activation of splenocytes.
American Psychological Association (APA)
Hedberg, Annica& Kanapathippillai, Premasany& Rekvig, Ole Petter& Fenton, Kristin Andreassen. 2013. LMW Heparin Prevents Increased Kidney Expression of Proinflammatory Mediators in (NZBxNZW)F1 Mice. Clinical and Developmental Immunology،Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-498375
Modern Language Association (MLA)
Hedberg, Annica…[et al.]. LMW Heparin Prevents Increased Kidney Expression of Proinflammatory Mediators in (NZBxNZW)F1 Mice. Clinical and Developmental Immunology No. 2013 (2013), pp.1-11.
https://search.emarefa.net/detail/BIM-498375
American Medical Association (AMA)
Hedberg, Annica& Kanapathippillai, Premasany& Rekvig, Ole Petter& Fenton, Kristin Andreassen. LMW Heparin Prevents Increased Kidney Expression of Proinflammatory Mediators in (NZBxNZW)F1 Mice. Clinical and Developmental Immunology. 2013. Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-498375
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-498375