Protective Effect of Melatonin Against Mitomycin C-Induced Genotoxic Damage in Peripheral Blood of Rats
Joint Authors
Lostalé, F.
Fuentes-Broto, Lorena
García, J. J.
Martínez-Ballarín, E.
Ortega-Gutiérrez, S.
López-Vicente, M.
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-10-20
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Mitomycin C (MMC) generates free radicals when metabolized.
We investigated the effect of melatonin against MMC-induced genotoxicity in polychromatic erythrocytes and MMC-induced lipid peroxidation in brain and liver homogenates.
Rats (N = 36) were classified into 4 groups: control, melatonin, MMC, and MMC + melatonin.
Melatonin and MMC doses of 10 mg/kg and 2 mg/kg, respectively, were injected intraperitoneally.
Peripheral blood samples were collected at 0, 24, 48, 72, and 96 hours posttreatment and homogenates were obtained at 96 hours posttreatment.
The number of micronucleated polychromatic erythrocytes (MN-PCE) per 1000 PCE was used as a genotoxic marker.
Malondialdehyde (MDA) plus 4-hydroxyalkenal (4-HDA) levels were used as an index of lipid peroxidation.
The MMC group showed a significant increase in MN-PCE at 24, 48, 72, and 96 hours that was significantly reduced with melatonin begin coadministrated.
No significant differences were found in lipid peroxidation.
Our results indicate that MMC-induced genotoxicity can be reduced by melatonin.
American Psychological Association (APA)
Ortega-Gutiérrez, S.& López-Vicente, M.& Lostalé, F.& Fuentes-Broto, Lorena& Martínez-Ballarín, E.& García, J. J.. 2009. Protective Effect of Melatonin Against Mitomycin C-Induced Genotoxic Damage in Peripheral Blood of Rats. Journal of Biomedicine and Biotechnology،Vol. 2009, no. 2009, pp.1-6.
https://search.emarefa.net/detail/BIM-498398
Modern Language Association (MLA)
Ortega-Gutiérrez, S.…[et al.]. Protective Effect of Melatonin Against Mitomycin C-Induced Genotoxic Damage in Peripheral Blood of Rats. Journal of Biomedicine and Biotechnology No. 2009 (2009), pp.1-6.
https://search.emarefa.net/detail/BIM-498398
American Medical Association (AMA)
Ortega-Gutiérrez, S.& López-Vicente, M.& Lostalé, F.& Fuentes-Broto, Lorena& Martínez-Ballarín, E.& García, J. J.. Protective Effect of Melatonin Against Mitomycin C-Induced Genotoxic Damage in Peripheral Blood of Rats. Journal of Biomedicine and Biotechnology. 2009. Vol. 2009, no. 2009, pp.1-6.
https://search.emarefa.net/detail/BIM-498398
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-498398
