Correlation between Agar Plate Screening and Solid-State Fermentation for the Prediction of Cellulase Production by Trichoderma Strains
Joint Authors
Florencio, Camila
Farinas, Cristiane Sanchez
Couri, Sonia
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-11-28
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
The viability of converting biomass into biofuels and chemicals still requires further development towards the reduction of the enzyme production costs.
Thus, there is a growing demand for the development of efficient procedures for selection of cellulase-producing microorganisms.
This work correlates qualitative screening using agar plate assays with quantitative measurements of cellulase production during cultivation under solid-state fermentation (SSF).
The initial screening step consisted of observation of the growth of 78 preselected strains of the genus Trichoderma on plates, using microcrystalline cellulose as carbon source.
The 49 strains that were able to grow on this substrate were then subjected to a second screening step using the Congo red test.
From this test it was possible to select 10 strains that presented the highest enzymatic indices (EI), with values ranging from 1.51 to 1.90.
SSF cultivations using sugarcane bagasse and wheat bran as substrates were performed using selected strains.
The CG 104NH strain presented the highest EGase activity (25.93 UI·g−1).
The EI results obtained in the screening procedure using plates were compared with cellulase production under SSF.
A correlation coefficient (R2) of 0.977 was obtained between the Congo red test and SSF, demonstrating that the two methodologies were in good agreement.
American Psychological Association (APA)
Florencio, Camila& Couri, Sonia& Farinas, Cristiane Sanchez. 2012. Correlation between Agar Plate Screening and Solid-State Fermentation for the Prediction of Cellulase Production by Trichoderma Strains. Enzyme Research،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-498603
Modern Language Association (MLA)
Florencio, Camila…[et al.]. Correlation between Agar Plate Screening and Solid-State Fermentation for the Prediction of Cellulase Production by Trichoderma Strains. Enzyme Research No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-498603
American Medical Association (AMA)
Florencio, Camila& Couri, Sonia& Farinas, Cristiane Sanchez. Correlation between Agar Plate Screening and Solid-State Fermentation for the Prediction of Cellulase Production by Trichoderma Strains. Enzyme Research. 2012. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-498603
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-498603