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Optimization of the Nutritional Parameters for Enhanced Production of B. subtilis SPB1 Biosurfactant in Submerged Culture Using Response Surface Methodology
Joint Authors
Chaabouni-Ellouze, Semia
Mnif, Ines
Chaabouni-Ellouze, Semia
Source
Biotechnology Research International
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-05-07
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Information Technology and Computer Science
Abstract EN
Nutritional requirements can contribute considerably to the production cost and the bioprocess economics.
Media optimisation using response surface methodology is one of the used methods to ameliorate the bioprocess economics.
In the present study, biosurfactant production by Bacillus subtilis SPB1 was effectively enhanced by response surface methodology.
A Plackett-Burman-based statistical screening procedure was adopted to determine the most important factor affecting lipopeptide production.
Eleven variables are screened and results show that glucose, K2HPO4, and urea concentrations influence the most biosurfactant production.
A Central Composite Design was conducted to optimize the three selected factors.
Statistical analyses of the data of model fitting were done by using NemrodW.
Results show a maximum predicted biosurfactant concentration of 2.93 (±0.32) g/L when using 15 g/L glucose, 6 g/L urea, and 1 g/L K2HPO4.
The predicted value is approximately 1.65 much higher than the original production determined by the conventional one-factor-at-a-time optimization method.
American Psychological Association (APA)
Mnif, Ines& Chaabouni-Ellouze, Semia& Chaabouni-Ellouze, Semia. 2012. Optimization of the Nutritional Parameters for Enhanced Production of B. subtilis SPB1 Biosurfactant in Submerged Culture Using Response Surface Methodology. Biotechnology Research International،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-498771
Modern Language Association (MLA)
Mnif, Ines…[et al.]. Optimization of the Nutritional Parameters for Enhanced Production of B. subtilis SPB1 Biosurfactant in Submerged Culture Using Response Surface Methodology. Biotechnology Research International No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-498771
American Medical Association (AMA)
Mnif, Ines& Chaabouni-Ellouze, Semia& Chaabouni-Ellouze, Semia. Optimization of the Nutritional Parameters for Enhanced Production of B. subtilis SPB1 Biosurfactant in Submerged Culture Using Response Surface Methodology. Biotechnology Research International. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-498771
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-498771