Analysis of the Virulence of an Atypical Enteropathogenic Escherichia coli Strain In Vitro and In Vivo and the Influence of Type Three Secretion System
Joint Authors
Vieira, Mônica Aparecida Midolli
Felipe-Silva, Aloísio
Liberatore, Ana M. A.
Knöbl, Terezinha
Sinigaglia-Coimbra, Rita
Ferreira, Claudete S. A.
Gomes, Tânia Aparecida Tardelli
Moreira, Fabiana C.
Sampaio, Suely C. F.
Hernandes, Rodrigo T.
Romão, Fabiano T.
Koh, Ivan H. J.
Ferreira, Antônio José Piantino
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-04-28
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Atypical enteropathogenic Escherichia coli (aEPEC) inject various effectors into intestinal cells through a type three secretion system (T3SS), causing attaching and effacing (A/E) lesions.
We investigated the role of T3SS in the ability of the aEPEC 1711-4 strain to interact with enterocytes in vitro (Caco-2 cells) and in vivo (rabbit ileal loops) and to translocate the rat intestinal mucosa in vivo.
A T3SS isogenic mutant strain was constructed, which showed marked reduction in the ability to associate and invade but not to persist inside Caco-2 cells.
After rabbit infection, only aEPEC 1711-4 was detected inside enterocytes at 8 and 24 hours pointing to a T3SS-dependent invasive potential in vivo.
In contrast to aEPEC 1711-4, the T3SS-deficient strain no longer produced A/E lesions or induced macrophage infiltration.
We also demonstrated that the ability of aEPEC 1711-4 to translocate through mesenteric lymph nodes to spleen and liver in a rat model depends on a functional T3SS, since a decreased number of T3SS mutant bacteria were recovered from extraintestinal sites.
These findings indicate that the full virulence potential of aEPEC 1711-4 depends on a functional T3SS, which contributes to efficient adhesion/invasion in vitro and in vivo and to bacterial translocation to extraintestinal sites.
American Psychological Association (APA)
Sampaio, Suely C. F.& Moreira, Fabiana C.& Liberatore, Ana M. A.& Vieira, Mônica Aparecida Midolli& Knöbl, Terezinha& Romão, Fabiano T.…[et al.]. 2014. Analysis of the Virulence of an Atypical Enteropathogenic Escherichia coli Strain In Vitro and In Vivo and the Influence of Type Three Secretion System. BioMed Research International،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-498947
Modern Language Association (MLA)
Sampaio, Suely C. F.…[et al.]. Analysis of the Virulence of an Atypical Enteropathogenic Escherichia coli Strain In Vitro and In Vivo and the Influence of Type Three Secretion System. BioMed Research International No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-498947
American Medical Association (AMA)
Sampaio, Suely C. F.& Moreira, Fabiana C.& Liberatore, Ana M. A.& Vieira, Mônica Aparecida Midolli& Knöbl, Terezinha& Romão, Fabiano T.…[et al.]. Analysis of the Virulence of an Atypical Enteropathogenic Escherichia coli Strain In Vitro and In Vivo and the Influence of Type Three Secretion System. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-498947
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-498947