A New Strategy to Find Targets for Anticancer Therapy : Chemokine CXCL14BRAK Is a Multifunctional Tumor Suppressor for Head and Neck Squamous Cell Carcinoma
Author
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-11-14
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
In order to find a suppressor(s) of tumor progression in vivo for head and neck squamous cell carcinoma (HNSCC), we searched for molecules downregulated in HNSCC cells when the cells were treated with epidermal growth factor (EGF), whose receptor is frequently overactivated in HNSCC.
The expression of BRAK, which is also known as CXC chemokine ligand 14 (CXCL14), was downregulated significantly by the treatment of HNSCC cells with EGF as observed by cDNA microarray analysis followed by reverse-transcriptase polymerase chain reaction analysis and western blotting.
The EGF effect on the expression of CXCL14/BRAK was attenuated by the copresence of inhibitors of the EGF receptor, MEK, and ERK.
The rate of tumor formation in vivo of BRAK-expressing vector-transfected tumor cells in athymic nude mice or SCID mice was significantly lower than that of mock vector-transfected ones.
In addition tumors formed in vivo by the BRAK-expressing cells were significantly smaller than those of the mock-transfected ones.
These results indicate that CXCL14/BRAK is a chemokine having suppressive activity toward tumor progression of HNSCC in vivo.
Our approach will be useful to find new target molecules to suppress progression of tumors of various origins in addition to HNSCC.
American Psychological Association (APA)
Hata, Ryu-Ichiro. 2012. A New Strategy to Find Targets for Anticancer Therapy : Chemokine CXCL14BRAK Is a Multifunctional Tumor Suppressor for Head and Neck Squamous Cell Carcinoma. ISRN Otolaryngology،Vol. 2012, no. 2012, pp.1-12.
https://search.emarefa.net/detail/BIM-498952
Modern Language Association (MLA)
Hata, Ryu-Ichiro. A New Strategy to Find Targets for Anticancer Therapy : Chemokine CXCL14BRAK Is a Multifunctional Tumor Suppressor for Head and Neck Squamous Cell Carcinoma. ISRN Otolaryngology No. 2012 (2012), pp.1-12.
https://search.emarefa.net/detail/BIM-498952
American Medical Association (AMA)
Hata, Ryu-Ichiro. A New Strategy to Find Targets for Anticancer Therapy : Chemokine CXCL14BRAK Is a Multifunctional Tumor Suppressor for Head and Neck Squamous Cell Carcinoma. ISRN Otolaryngology. 2012. Vol. 2012, no. 2012, pp.1-12.
https://search.emarefa.net/detail/BIM-498952
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-498952