Effects of Immunomodulatory Substances on Phagocytosis of Aβ1–42 by Human Microglia
Joint Authors
Hjorth, Erik
Weiner, Howard L.
Schultzberg, Marianne
Frenkel, Dan
Source
International Journal of Alzheimer's Disease
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-18, 18 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-05-20
Country of Publication
Egypt
No. of Pages
18
Main Subjects
Abstract EN
Glial activation and increased inflammation characterize neuropathology in Alzheimer's disease (AD).
The aim was to develop a model for studying phagocytosis of β-amyloid (Aβ) peptide by human microglia and to test effects thereupon by immunomodulatory substances.
Human CHME3 microglia showed intracellular Aβ1–42 colocalized with lysosome-associated membrane protein-2, indicating phagocytosis.
This was increased by interferon-γ, and to a lesser degree with Protollin, a proteosome-based adjuvant.
Secretion of brain-derived neurotrophic factor (BDNF) was decreased by Aβ1–42 and by interferon-γ and interleukin-1β.
These cytokines, but not Aβ1–42, stimulated interleukin-6 release.
Microglia which phagocytosed Aβ1–42 exhibited a higher degree of expression of interleukin-1 receptor type I and inducible nitric oxide synthase.
In conclusion, we show that human microglia are able to phagocytose Aβ1–42 and that this is associated with expression of inflammatory markers.
Aβ1–42 and interferon-γ decreased BDNF secretion suggesting a new neuropathological role for Aβ1–42 and the inflammation accompanying AD.
American Psychological Association (APA)
Hjorth, Erik& Frenkel, Dan& Weiner, Howard L.& Schultzberg, Marianne. 2010. Effects of Immunomodulatory Substances on Phagocytosis of Aβ1–42 by Human Microglia. International Journal of Alzheimer's Disease،Vol. 2010, no. 2010, pp.1-18.
https://search.emarefa.net/detail/BIM-499032
Modern Language Association (MLA)
Hjorth, Erik…[et al.]. Effects of Immunomodulatory Substances on Phagocytosis of Aβ1–42 by Human Microglia. International Journal of Alzheimer's Disease No. 2010 (2010), pp.1-18.
https://search.emarefa.net/detail/BIM-499032
American Medical Association (AMA)
Hjorth, Erik& Frenkel, Dan& Weiner, Howard L.& Schultzberg, Marianne. Effects of Immunomodulatory Substances on Phagocytosis of Aβ1–42 by Human Microglia. International Journal of Alzheimer's Disease. 2010. Vol. 2010, no. 2010, pp.1-18.
https://search.emarefa.net/detail/BIM-499032
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-499032