Proteomic Analysis of Bacillus thuringiensis Strain 4.0718 at Different Growth Phases
Joint Authors
Yin, Jia
Xia, Liqiu
Sun, Yunjun
Yuan, Can
Ding, Xuezhi
Li, Xiaohui
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-04-29
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Information Technology and Computer Science
Abstract EN
The growth process of Bacillus thuringiensis Bt4.0718 strain was studied using proteomic technologies.
The proteins of Bt whole cells at three phases—middle vegetative, early sporulation, and late sporulation—were extracted with lysis buffer, followed with separation by 2-DE and identified by MALDI-TOF/TOF MS.
Bioactive factors such as insecticidal crystal proteins (ICPs) including Cry1Ac(3), Cry2Aa, and BTRX28, immune inhibitor (InhA), and InhA precursor were identified.
InhA started to express at the middle vegetative phase, suggesting its contribution to the survival of Bt in the host body.
At the early sporulation phase, ICPs started their expression.
CotJC, OppA, ORF1, and SpoIVA related to the formation of crystals and spores were identified, the expression characteristics of which ensured the stable formation of crystals and spores.
This study provides an important foundation for further exploration of the stable expression of ICPs, the smooth formation of crystals, and the construction of recombinant strains.
American Psychological Association (APA)
Li, Xiaohui& Ding, Xuezhi& Xia, Liqiu& Sun, Yunjun& Yuan, Can& Yin, Jia. 2012. Proteomic Analysis of Bacillus thuringiensis Strain 4.0718 at Different Growth Phases. The Scientific World Journal،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-499064
Modern Language Association (MLA)
Li, Xiaohui…[et al.]. Proteomic Analysis of Bacillus thuringiensis Strain 4.0718 at Different Growth Phases. The Scientific World Journal No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-499064
American Medical Association (AMA)
Li, Xiaohui& Ding, Xuezhi& Xia, Liqiu& Sun, Yunjun& Yuan, Can& Yin, Jia. Proteomic Analysis of Bacillus thuringiensis Strain 4.0718 at Different Growth Phases. The Scientific World Journal. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-499064
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-499064