Rapid Degradation of Hfq-Free RyhB in Yersinia pestis by PNPase Independent of Putative Ribonucleolytic Complexes
Joint Authors
Deng, Zhongliang
Wang, Xiaoyi
Zhou, Dongsheng
Yang, Ruifu
Liu, Zizhong
Han, Yanping
Bi, Yujing
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-04-10
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
The RNA chaperone Hfq in bacteria stabilizes sRNAs by protecting them from the attack of ribonucleases.
Upon release from Hfq, sRNAs are preferably degraded by PNPase.
PNPase usually forms multienzyme ribonucleolytic complexes with endoribonuclease E and/or RNA helicase RhlB to facilitate the degradation of the structured RNA.
However, whether PNPase activity on Hfq-free sRNAs is associated with the assembly of RNase E or RhlB has yet to be determined.
Here we examined the roles of the main endoribonucleases, exoribonucleases, and ancillary RNA-modifying enzymes in the degradation of Y.
pestis RyhB in the absence of Hfq.
Expectedly, the transcript levels of both RyhB1 and RyhB2 increase only after inactivating PNPase, which confirms the importance of PNPase in sRNA degradation.
By contrast, the signal of RyhB becomes barely perceptible after inactivating of RNase III, which may be explained by the increase in PNPase levels resulting from the exemption of pnp mRNA from RNase III processing.
No significant changes are observed in RyhB stability after deletion of either the PNPase-binding domain of RNase E or rhlB.
Therefore, PNPase acts as a major enzyme of RyhB degradation independent of PNPase-containing RNase E and RhlB assembly in the absence of Hfq.
American Psychological Association (APA)
Deng, Zhongliang& Liu, Zizhong& Bi, Yujing& Wang, Xiaoyi& Zhou, Dongsheng& Yang, Ruifu…[et al.]. 2014. Rapid Degradation of Hfq-Free RyhB in Yersinia pestis by PNPase Independent of Putative Ribonucleolytic Complexes. BioMed Research International،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-499072
Modern Language Association (MLA)
Deng, Zhongliang…[et al.]. Rapid Degradation of Hfq-Free RyhB in Yersinia pestis by PNPase Independent of Putative Ribonucleolytic Complexes. BioMed Research International No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-499072
American Medical Association (AMA)
Deng, Zhongliang& Liu, Zizhong& Bi, Yujing& Wang, Xiaoyi& Zhou, Dongsheng& Yang, Ruifu…[et al.]. Rapid Degradation of Hfq-Free RyhB in Yersinia pestis by PNPase Independent of Putative Ribonucleolytic Complexes. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-499072
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-499072