Curcumin Pretreatment Induces Nrf2 and an Antioxidant Response and Prevents Hemin-Induced Toxicity in Primary Cultures of Cerebellar Granule Neurons of Rats
Joint Authors
Medina-Campos, Omar Noel
Guzmán-Beltrán, Silvia
Pedraza-Chaverri, José
González-Reyes, Susana
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-12-22
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
Curcumin is a bifunctional antioxidant derived from Curcuma longa.
This study identifies curcumin as a neuroprotectant against hemin-induced damage in primary cultures of cerebellar granule neurons (CGNs) of rats.
Hemin, the oxidized form of heme, is a highly reactive compound that induces cellular injury.
Pretreatment of CGNs with 5–30 μM curcumin effectively increased by 2.3–4.9 fold heme oxygenase-1 (HO-1) expression and by 5.6–14.3-fold glutathione (GSH) levels.
Moreover, 15 μM curcumin attenuated by 55% the increase in reactive oxygen species (ROS) production, by 94% the reduction of GSH/glutathione disulfide (GSSG) ratio, and by 49% the cell death induced by hemin.
The inhibition of heme oxygenase system or GSH synthesis with tin mesoporphyrin and buthionine sulfoximine, respectively, suppressed the protective effect of curcumin against hemin-induced toxicity.
These data strongly suggest that HO-1 and GSH play a major role in the protective effect of curcumin.
Furthermore, it was found that 24 h of incubation with curcumin increases by 1.4-, 2.3-, and 5.2-fold the activity of glutathione reductase, glutathione S-transferase and superoxide dismutase, respectively.
Additionally, it was found that curcumin was capable of inducing nuclear factor (erythroid-derived 2)-like 2 (Nrf2) translocation into the nucleus.
These data suggest that the pretreatment with curcumin induces Nrf2 and an antioxidant response that may play an important role in the protective effect of this antioxidant against hemin-induced neuronal death.
American Psychological Association (APA)
González-Reyes, Susana& Guzmán-Beltrán, Silvia& Medina-Campos, Omar Noel& Pedraza-Chaverri, José. 2013. Curcumin Pretreatment Induces Nrf2 and an Antioxidant Response and Prevents Hemin-Induced Toxicity in Primary Cultures of Cerebellar Granule Neurons of Rats. Oxidative Medicine and Cellular Longevity،Vol. 2013, no. 2013, pp.1-14.
https://search.emarefa.net/detail/BIM-499119
Modern Language Association (MLA)
González-Reyes, Susana…[et al.]. Curcumin Pretreatment Induces Nrf2 and an Antioxidant Response and Prevents Hemin-Induced Toxicity in Primary Cultures of Cerebellar Granule Neurons of Rats. Oxidative Medicine and Cellular Longevity No. 2013 (2013), pp.1-14.
https://search.emarefa.net/detail/BIM-499119
American Medical Association (AMA)
González-Reyes, Susana& Guzmán-Beltrán, Silvia& Medina-Campos, Omar Noel& Pedraza-Chaverri, José. Curcumin Pretreatment Induces Nrf2 and an Antioxidant Response and Prevents Hemin-Induced Toxicity in Primary Cultures of Cerebellar Granule Neurons of Rats. Oxidative Medicine and Cellular Longevity. 2013. Vol. 2013, no. 2013, pp.1-14.
https://search.emarefa.net/detail/BIM-499119
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-499119