Development and Validation of RP-HPLC Method for Quantitative Estimation of Vinpocetine in Pure and Pharmaceutical Dosage Forms
Joint Authors
Arefeen, Shamsul
Roy, Sumon
Bhadra, Subrata
Rouf, Abu Shara Shamsur
Das, Sreedam Chandra
Source
Chromatography Research International
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-11-15
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
A simple, precise, specific, and accurate reversed phase high performance liquid chromatographic (RP-HPLC) method was developed and validated for determination of vinpocetine in pure and pharmaceutical dosage forms.
The different analytical performance parameters such as linearity, accuracy, specificity, precision, and sensitivity (limit of detection and limit of quantitation) were determined according to International Conference on Harmonization ICH Q2 (R1) guidelines.
RP-HPLC was conducted on Zorbax C18 (150 mm length × 4.6 mm ID, 5 μm) column.
The mobile phase was consisting of buffer (containing 1.54% w/v ammonium acetate solution) and acetonitrile in the ratio (40 : 60, v/v), and the flow rate was maintained at 1.0 mLmin−1.
Vinpocetine was monitored using Agilent 1200 series equipped with photo diode array detector (λ = 280 nm).
Linearity was observed in concentration range of 160–240 μgmL−1, and correlation coefficient was found excellent (R2 = 0.999).
All the system suitability parameters were found within the range.
The proposed method is rapid, cost-effective and can be used as a quality-control tool for routine quantitative analysis of vinpocetine in pure and pharmaceutical dosage forms.
American Psychological Association (APA)
Bhadra, Subrata& Das, Sreedam Chandra& Roy, Sumon& Arefeen, Shamsul& Rouf, Abu Shara Shamsur. 2011. Development and Validation of RP-HPLC Method for Quantitative Estimation of Vinpocetine in Pure and Pharmaceutical Dosage Forms. Chromatography Research International،Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-499146
Modern Language Association (MLA)
Bhadra, Subrata…[et al.]. Development and Validation of RP-HPLC Method for Quantitative Estimation of Vinpocetine in Pure and Pharmaceutical Dosage Forms. Chromatography Research International No. 2011 (2011), pp.1-7.
https://search.emarefa.net/detail/BIM-499146
American Medical Association (AMA)
Bhadra, Subrata& Das, Sreedam Chandra& Roy, Sumon& Arefeen, Shamsul& Rouf, Abu Shara Shamsur. Development and Validation of RP-HPLC Method for Quantitative Estimation of Vinpocetine in Pure and Pharmaceutical Dosage Forms. Chromatography Research International. 2011. Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-499146
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-499146