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Connexin 43 Expression on Peripheral Blood Eosinophils : Role of Gap Junctions in Transendothelial Migration
Joint Authors
Mahmudi-Azer, Salahaddin
Abel, Melanie
Ilarraza, Ramses
Befus, A. Dean
Vliagoftis, Harissios
Adamko, Darryl
Ebeling, Cory
Moqbel, Redwan
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-06
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Eosinophils circulate in the blood and are recruited in tissues during allergic inflammation.
Gap junctions mediate direct communication between adjacent cells and may represent a new way of communication between immune cells distinct from communication through cytokines and chemokines.
We characterized the expression of connexin (Cx)43 by eosinophils isolated from atopic individuals using RT-PCR, Western blotting, and confocal microscopy and studied the biological functions of gap junctions on eosinophils.
The formation of functional gap junctions was evaluated measuring dye transfer using flow cytometry.
The role of gap junctions on eosinophil transendothelial migration was studied using the inhibitor 18-a-glycyrrhetinic acid.
Peripheral blood eosinophils express Cx43 mRNA and protein.
Cx43 is localized not only in the cytoplasm but also on the plasma membrane.
The membrane impermeable dye BCECF transferred from eosinophils to epithelial or endothelial cells following coculture in a dose and time dependent fashion.
The gap junction inhibitors 18-a-glycyrrhetinic acid and octanol did not have a significant effect on dye transfer but reduced dye exit from eosinophils.
The gap junction inhibitor 18-a-glycyrrhetinic acid inhibited eosinophil transendothelial migration in a dose dependent manner.
Thus, eosinophils from atopic individuals express Cx43 constitutively and Cx43 may play an important role in eosinophil transendothelial migration and function in sites of inflammation.
American Psychological Association (APA)
Vliagoftis, Harissios& Ebeling, Cory& Ilarraza, Ramses& Mahmudi-Azer, Salahaddin& Abel, Melanie& Adamko, Darryl…[et al.]. 2014. Connexin 43 Expression on Peripheral Blood Eosinophils : Role of Gap Junctions in Transendothelial Migration. BioMed Research International،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-499282
Modern Language Association (MLA)
Vliagoftis, Harissios…[et al.]. Connexin 43 Expression on Peripheral Blood Eosinophils : Role of Gap Junctions in Transendothelial Migration. BioMed Research International No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-499282
American Medical Association (AMA)
Vliagoftis, Harissios& Ebeling, Cory& Ilarraza, Ramses& Mahmudi-Azer, Salahaddin& Abel, Melanie& Adamko, Darryl…[et al.]. Connexin 43 Expression on Peripheral Blood Eosinophils : Role of Gap Junctions in Transendothelial Migration. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-499282
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-499282