High-Frequency Vibration Treatment of Human Bone Marrow Stromal Cells Increases Differentiation toward Bone Tissue
Joint Authors
Visai, Livia
Cusella De Angelis, Maria Gabriella
Prè, D.
Benedetti, L.
Imbriani, M.
Magenes, Giovanni
Ceccarelli, G.
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-03-25
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
In order to verify whether differentiation of adult stem cells toward bone tissue is promoted by high-frequency vibration (HFV), bone marrow stromal cells (BMSCs) were mechanically stimulated with HFV (30 Hz) for 45 minutes a day for 21 or 40 days.
Cells were seeded in osteogenic medium, which enhances differentiation towards bone tissue.
The effects of the mechanical treatment on differentiation were measured by Alizarin Red test, (q) real-time PCR, and protein content of the extracellular matrix.
In addition, we analyzed the proliferation rate and apoptosis of BMSC subjected to mechanical stimulation.
A strong increase in all parameters characterizing differentiation was observed.
Deposition of calcium was almost double in the treated samples; the expression of genes involved in later differentiation was significantly increased and protein content was higher for all osteogenic proteins.
Lastly, proliferation results indicated that stimulated BMSCs have a decreased growth rate in comparison with controls, but both treated and untreated cells do not enter the apoptosis process.
These findings could reduce the gap between research and clinical application for bone substitutes derived from patient cells by improving the differentiation protocol for autologous cells and a further implant of the bone graft into the patient.
American Psychological Association (APA)
Prè, D.& Ceccarelli, G.& Visai, Livia& Benedetti, L.& Imbriani, M.& Cusella De Angelis, Maria Gabriella…[et al.]. 2013. High-Frequency Vibration Treatment of Human Bone Marrow Stromal Cells Increases Differentiation toward Bone Tissue. Bone Marrow Research،Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-499295
Modern Language Association (MLA)
Prè, D.…[et al.]. High-Frequency Vibration Treatment of Human Bone Marrow Stromal Cells Increases Differentiation toward Bone Tissue. Bone Marrow Research No. 2013 (2013), pp.1-13.
https://search.emarefa.net/detail/BIM-499295
American Medical Association (AMA)
Prè, D.& Ceccarelli, G.& Visai, Livia& Benedetti, L.& Imbriani, M.& Cusella De Angelis, Maria Gabriella…[et al.]. High-Frequency Vibration Treatment of Human Bone Marrow Stromal Cells Increases Differentiation toward Bone Tissue. Bone Marrow Research. 2013. Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-499295
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-499295