Amperometric Detection of Bacillus anthracis Spores : A Portable, Low-Cost Approach to the ELISA
Joint Authors
Hew, Brian E.
Peckham, Gabriel D.
Holdaway, Charlie
Jen, Michael
Waller, David F.
Source
International Journal of Electrochemistry
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-09-09
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Antibody-based detection assays are generally robust, a desirable characteristic for in-the-field use.
However, to quantify the colorimetric or fluorescent signal, these assays require expensive and fragile instruments which are ill-suited to in-the-field use.
Lateral flow devices (LFDs) circumvent these barriers to portability but suffer from poor sensitivity and subjective interpretation.
Here, an antibody-based method for detecting Bacillus anthracis spores via amperometric signal generation is compared to ELISA and LFDs.
This amperometric immunoassay uses antibody conjugated to magnetic beads and glucose oxidase (GOX) along with the electron mediator 2, 6-dichlorophenolindophenol (DCPIP) for production of a measurable current from a 0.4 V bias voltage.
With similar sensitivity to ELISA, the assay can be completed in about 75 minutes while being completely powered and operated from a laptop computer.
Immunoassay amperometry holds promise for bringing low-cost, quantitative detection of hazardous agents to the field.
American Psychological Association (APA)
Peckham, Gabriel D.& Hew, Brian E.& Waller, David F.& Holdaway, Charlie& Jen, Michael. 2013. Amperometric Detection of Bacillus anthracis Spores : A Portable, Low-Cost Approach to the ELISA. International Journal of Electrochemistry،Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-499299
Modern Language Association (MLA)
Peckham, Gabriel D.…[et al.]. Amperometric Detection of Bacillus anthracis Spores : A Portable, Low-Cost Approach to the ELISA. International Journal of Electrochemistry No. 2013 (2013), pp.1-6.
https://search.emarefa.net/detail/BIM-499299
American Medical Association (AMA)
Peckham, Gabriel D.& Hew, Brian E.& Waller, David F.& Holdaway, Charlie& Jen, Michael. Amperometric Detection of Bacillus anthracis Spores : A Portable, Low-Cost Approach to the ELISA. International Journal of Electrochemistry. 2013. Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-499299
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-499299