Identification of Human Serum Peptides in Fourier Transform Ion Cyclotron Resonance Precision Profiles
Joint Authors
van der Burgt, Yuri E. M.
Mesker, Wilma E.
Nicolardi, Simone
Dalebout, Hans
Tollenaar, Rob A. E. M.
Bladergroen, Marco R.
Deelder, André M.
Source
International Journal of Proteomics
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-05-22
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
The continuous efforts to find new prognostic or diagnostic biomarkers have stimulated the use of mass spectrometry (MS) profiles in a clinical setting.
In the early days (about one decade ago), a single low-resolution mass spectrum derived from an individual’s body fluid was used for comparative studies.
However, a peptide profile of a complex mixture is most informative when recorded on an ultrahigh resolution instrument such as a Fourier transform ion cyclotron resonance (FTICR) mass spectrometer.
In this study we show the benefits of the ultrahigh resolving power and the high mass accuracy and precision provided by an FTICR mass spectrometer equipped with a 15-tesla magnet.
The ultrahigh-resolution data not only allow assignment of fragment ions with high charge states (4+, 5+) but also enhance confidence of human serum peptide identifications from tandem MS experiments.
This is exemplified with collision-induced dissociation (CID) and electron transfer dissociation (ETD) data of middle-down-sized endogenous or protein-breakdown peptides that are of interest in biomarker discovery studies.
American Psychological Association (APA)
Nicolardi, Simone& Dalebout, Hans& Bladergroen, Marco R.& Mesker, Wilma E.& Tollenaar, Rob A. E. M.& Deelder, André M.…[et al.]. 2012. Identification of Human Serum Peptides in Fourier Transform Ion Cyclotron Resonance Precision Profiles. International Journal of Proteomics،Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-499350
Modern Language Association (MLA)
Nicolardi, Simone…[et al.]. Identification of Human Serum Peptides in Fourier Transform Ion Cyclotron Resonance Precision Profiles. International Journal of Proteomics No. 2012 (2012), pp.1-6.
https://search.emarefa.net/detail/BIM-499350
American Medical Association (AMA)
Nicolardi, Simone& Dalebout, Hans& Bladergroen, Marco R.& Mesker, Wilma E.& Tollenaar, Rob A. E. M.& Deelder, André M.…[et al.]. Identification of Human Serum Peptides in Fourier Transform Ion Cyclotron Resonance Precision Profiles. International Journal of Proteomics. 2012. Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-499350
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-499350