Synthesis of Specifically Modified Oligonucleotides for Application in Structural and Functional Analysis of RNA
Joint Authors
Springstubbe, Danilo
Rublack, Nico
Appel, Bettina
Strohbach, Denise
Müller, Sabine
Nguyen, Hien
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-19, 19 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-10-12
Country of Publication
Egypt
No. of Pages
19
Main Subjects
Abstract EN
Nowadays, RNA synthesis has become an essential tool not only in the field of molecular biology and medicine, but also in areas like molecular diagnostics and material sciences.
Beyond synthetic RNAs for antisense, aptamer, ribozyme, and siRNA technologies, oligoribonucleotides carrying site-specific modifications for structure and function studies are needed.
This often requires labeling of the RNA with a suitable spectroscopic reporter group.
Herein, we describe the synthesis of functionalized monomer building blocks that upon incorporation in RNA allow for selective reaction with a specific reporter or functional entity.
In particular, we report on the synthesis of 5′-O-dimethoxytrityl-2′-O-tert-butyldimethylsilyl protected 3′-O-phosphoramidites of nucleosides that carry amino linkers of different lengths and flexibility at the heterocyclic base, their incorporation in a variety of RNAs, and postsynthetic conjugation with fluorescent dyes and nitroxide spin labels.
Further, we show the synthesis of a flavine mononucleotide-N-hydroxy-succinimidyl ester and its conjugation to amino functionalized RNA.
American Psychological Association (APA)
Rublack, Nico& Nguyen, Hien& Appel, Bettina& Springstubbe, Danilo& Strohbach, Denise& Müller, Sabine. 2011. Synthesis of Specifically Modified Oligonucleotides for Application in Structural and Functional Analysis of RNA. Journal of Nucleic Acids،Vol. 2011, no. 2011, pp.1-19.
https://search.emarefa.net/detail/BIM-499454
Modern Language Association (MLA)
Rublack, Nico…[et al.]. Synthesis of Specifically Modified Oligonucleotides for Application in Structural and Functional Analysis of RNA. Journal of Nucleic Acids No. 2011 (2011), pp.1-19.
https://search.emarefa.net/detail/BIM-499454
American Medical Association (AMA)
Rublack, Nico& Nguyen, Hien& Appel, Bettina& Springstubbe, Danilo& Strohbach, Denise& Müller, Sabine. Synthesis of Specifically Modified Oligonucleotides for Application in Structural and Functional Analysis of RNA. Journal of Nucleic Acids. 2011. Vol. 2011, no. 2011, pp.1-19.
https://search.emarefa.net/detail/BIM-499454
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-499454