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Stimulation of DNA Glycosylase Activities by XPC Protein Complex : Roles of Protein-Protein Interactions
Joint Authors
Uchimura, Yasuhiro
Saitoh, Hisato
Hanaoka, Fumio
Shimizu, Yuichiro
Sugasawa, Kaoru
Dohmae, Naoshi
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-07-25
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
We showed that XPC complex, which is a DNA damage detector for nucleotide excision repair, stimulates activity of thymine DNA glycosylase (TDG) that initiates base excision repair.
XPC appeared to facilitate the enzymatic turnover of TDG by promoting displacement from its own product abasic site, although the precise mechanism underlying this stimulation has not been clarified.
Here we show that XPC has only marginal effects on the activity of E.
coli TDG homolog (EcMUG), which remains bound to the abasic site like human TDG but does not significantly interacts with XPC.
On the contrary, XPC significantly stimulates the activities of sumoylated TDG and SMUG1, both of which exhibit quite different enzymatic kinetics from unmodified TDG but interact with XPC.
These results point to importance of physical interactions for stimulation of DNA glycosylases by XPC and have implications in the molecular mechanisms underlying mutagenesis and carcinogenesis in XP-C patients.
American Psychological Association (APA)
Shimizu, Yuichiro& Uchimura, Yasuhiro& Dohmae, Naoshi& Saitoh, Hisato& Hanaoka, Fumio& Sugasawa, Kaoru. 2010. Stimulation of DNA Glycosylase Activities by XPC Protein Complex : Roles of Protein-Protein Interactions. Journal of Nucleic Acids،Vol. 2010, no. 2010, pp.1-12.
https://search.emarefa.net/detail/BIM-499488
Modern Language Association (MLA)
Shimizu, Yuichiro…[et al.]. Stimulation of DNA Glycosylase Activities by XPC Protein Complex : Roles of Protein-Protein Interactions. Journal of Nucleic Acids No. 2010 (2010), pp.1-12.
https://search.emarefa.net/detail/BIM-499488
American Medical Association (AMA)
Shimizu, Yuichiro& Uchimura, Yasuhiro& Dohmae, Naoshi& Saitoh, Hisato& Hanaoka, Fumio& Sugasawa, Kaoru. Stimulation of DNA Glycosylase Activities by XPC Protein Complex : Roles of Protein-Protein Interactions. Journal of Nucleic Acids. 2010. Vol. 2010, no. 2010, pp.1-12.
https://search.emarefa.net/detail/BIM-499488
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-499488