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Norovirus Narita 104 Virus-Like Particles Expressed in Nicotiana benthamiana Induce Serum and Mucosal Immune Responses
Joint Authors
Herbst-Kralovetz, Melissa M.
Mason, Hugh S.
Mathew, Lolita George
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-11
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Narita 104 virus is a human pathogen belonging to the norovirus (family Caliciviridae) genogroup II.
Noroviruses cause epidemic gastroenteritis worldwide.
To explore the potential of developing a plant-based vaccine, a plant optimized gene encoding Narita 104 virus capsid protein (NaVCP) was expressed transiently in Nicotiana benthamiana using a tobacco mosaic virus expression system.
NaVCP accumulated up to approximately 0.3 mg/g fresh weight of leaf at 4 days postinfection.
Initiation of hypersensitive response-like symptoms followed by tissue necrosis necessitated a brief infection time and was a significant factor limiting expression.
Transmission electron microscopy of plant-derived NaVCP confirmed the presence of fully assembled virus-like particles (VLPs).
In this study, an optimized method to express and partially purify NaVCP is described.
Further, partially purified NaVCP was used to immunize mice by intranasal delivery and generated significant mucosal and serum antibody responses.
Thus, plant-derived Narita 104 VLPs have potential for use as a candidate subunit vaccine or as a component of a multivalent subunit vaccine, along with other genotype-specific plant-derived VLPs.
American Psychological Association (APA)
Mathew, Lolita George& Herbst-Kralovetz, Melissa M.& Mason, Hugh S.. 2014. Norovirus Narita 104 Virus-Like Particles Expressed in Nicotiana benthamiana Induce Serum and Mucosal Immune Responses. BioMed Research International،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-499668
Modern Language Association (MLA)
Mathew, Lolita George…[et al.]. Norovirus Narita 104 Virus-Like Particles Expressed in Nicotiana benthamiana Induce Serum and Mucosal Immune Responses. BioMed Research International No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-499668
American Medical Association (AMA)
Mathew, Lolita George& Herbst-Kralovetz, Melissa M.& Mason, Hugh S.. Norovirus Narita 104 Virus-Like Particles Expressed in Nicotiana benthamiana Induce Serum and Mucosal Immune Responses. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-499668
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-499668