Error-Prone Translesion DNA Synthesis by Escherichia coli DNA Polymerase IV (DinB) on Templates Containing 1,2-dihydro-2-oxoadenine
Joint Authors
Yonekura, Shin-Ichiro
Zhang-Akiyama, Qiu-Mei
Yonei, Shuji
Sugiyama, Hiroshi
Gruz, Petr
Hori, Masaki
Nohmi, Takehiko
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-09-26
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Escherichia coli DNA polymerase IV (Pol IV) is involved in bypass replication of damaged bases in DNA.
Reactive oxygen species (ROS) are generated continuously during normal metabolism and as a result of exogenous stress such as ionizing radiation.
ROS induce various kinds of base damage in DNA.
It is important to examine whether Pol IV is able to bypass oxidatively damaged bases.
In this study, recombinant Pol IV was incubated with oligonucleotides containing thymine glycol (dTg), 5-formyluracil (5-fodU), 5-hydroxymethyluracil (5-hmdU), 7,8-dihydro-8-oxoguanine (8-oxodG) and 1,2-dihydro-2-oxoadenine (2-oxodA).
Primer extension assays revealed that Pol IV preferred to insert dATP opposite 5-fodU and 5-hmdU, while it inefficiently inserted nucleotides opposite dTg.
Pol IV inserted dCTP and dATP opposite 8-oxodG, while the ability was low.
It inserted dCTP more effectively than dTTP opposite 2-oxodA.
Pol IV's ability to bypass these lesions decreased in the order: 2-oxodA > 5-fodU~5-hmdU > 8-oxodG > dTg.
The fact that Pol IV preferred to insert dCTP opposite 2-oxodA suggests the mutagenic potential of 2-oxodA leading to A:T→G:C transitions.
Hydrogen peroxide caused an ~2-fold increase in A:T→G:C mutations in E.
coli, while the increase was significantly greater in E.
coli overexpressing Pol IV.
These results indicate that Pol IV may be involved in ROS-enhanced A:T→G:C mutations.
American Psychological Association (APA)
Hori, Masaki& Yonekura, Shin-Ichiro& Nohmi, Takehiko& Gruz, Petr& Sugiyama, Hiroshi& Yonei, Shuji…[et al.]. 2010. Error-Prone Translesion DNA Synthesis by Escherichia coli DNA Polymerase IV (DinB) on Templates Containing 1,2-dihydro-2-oxoadenine. Journal of Nucleic Acids،Vol. 2010, no. 2010, pp.1-10.
https://search.emarefa.net/detail/BIM-499672
Modern Language Association (MLA)
Hori, Masaki…[et al.]. Error-Prone Translesion DNA Synthesis by Escherichia coli DNA Polymerase IV (DinB) on Templates Containing 1,2-dihydro-2-oxoadenine. Journal of Nucleic Acids No. 2010 (2010), pp.1-10.
https://search.emarefa.net/detail/BIM-499672
American Medical Association (AMA)
Hori, Masaki& Yonekura, Shin-Ichiro& Nohmi, Takehiko& Gruz, Petr& Sugiyama, Hiroshi& Yonei, Shuji…[et al.]. Error-Prone Translesion DNA Synthesis by Escherichia coli DNA Polymerase IV (DinB) on Templates Containing 1,2-dihydro-2-oxoadenine. Journal of Nucleic Acids. 2010. Vol. 2010, no. 2010, pp.1-10.
https://search.emarefa.net/detail/BIM-499672
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-499672