The Biosynthesis of the Molybdenum Cofactor in Escherichia coli and Its Connection to FeS Cluster Assembly and the Thiolation of tRNA
Author
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-21, 21 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-04-29
Country of Publication
Egypt
No. of Pages
21
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
The thiolation of biomolecules is a complex process that involves the activation of sulfur.
The L-cysteine desulfurase IscS is the main sulfur mobilizing protein in Escherichia coli that provides the sulfur from L-cysteine to several important biomolecules in the cell such as iron sulfur (FeS) clusters, molybdopterin (MPT), thiamine, and thionucleosides of tRNA.
Various proteins mediate the transfer of sulfur from IscS to various biomolecules using different interaction partners.
A direct connection between the sulfur-containing molecules FeS clusters, thiolated tRNA, and the molybdenum cofactor (Moco) has been identified.
The first step of Moco biosynthesis involves the conversion of 5′GTP to cyclic pyranopterin monophosphate (cPMP), a reaction catalyzed by a FeS cluster containing protein.
Formed cPMP is further converted to MPT by insertion of two sulfur atoms.
The sulfur for this reaction is provided by the L-cysteine desulfurase IscS in addition to the involvement of the TusA protein.
TusA is also involved in the sulfur transfer for the thiolation of tRNA.
This review will describe the biosynthesis of Moco in E.
coli in detail and dissects the sulfur transfer pathways for Moco and tRNA and their connection to FeS cluster biosynthesis.
American Psychological Association (APA)
Leimkühler, Silke. 2014. The Biosynthesis of the Molybdenum Cofactor in Escherichia coli and Its Connection to FeS Cluster Assembly and the Thiolation of tRNA. Advances in Biology،Vol. 2014, no. 2014, pp.1-21.
https://search.emarefa.net/detail/BIM-499754
Modern Language Association (MLA)
Leimkühler, Silke. The Biosynthesis of the Molybdenum Cofactor in Escherichia coli and Its Connection to FeS Cluster Assembly and the Thiolation of tRNA. Advances in Biology No. 2014 (2014), pp.1-21.
https://search.emarefa.net/detail/BIM-499754
American Medical Association (AMA)
Leimkühler, Silke. The Biosynthesis of the Molybdenum Cofactor in Escherichia coli and Its Connection to FeS Cluster Assembly and the Thiolation of tRNA. Advances in Biology. 2014. Vol. 2014, no. 2014, pp.1-21.
https://search.emarefa.net/detail/BIM-499754
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-499754