Specific Silencing of L392V PSEN1 Mutant Allele by RNA Interference
Joint Authors
Sochacka, Elzbieta
Nacmias, Benedetta
Bagnoli, Silvia
Nawrot, Barbara
Kazmierczak-Baranska, Julia
Sierant, Malgorzata
Sorbi, Sandro
Paduszynska, Alina
Source
International Journal of Alzheimer's Disease
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-04-07
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
RNA interference (RNAi) technology provides a powerful molecular tool to reduce an expression of selected genes in eukaryotic cells.
Short interfering RNAs (siRNAs) are the effector molecules that trigger RNAi.
Here, we describe siRNAs that discriminate between the wild type and mutant (1174 C→G) alleles of human Presenilin1 gene (PSEN1).
This mutation, resulting in L392V PSEN1 variant, contributes to early onset familial Alzheimer's disease.
Using the dual fluorescence assay, flow cytometry and fluorescent microscopy we identified positions 8th–11th, within the central part of the antisense strand, as the most sensitive to mismatches.
2-Thiouridine chemical modification introduced at the 3′-end of the antisense strand improved the allele discrimination, but wobble base pairing adjacent to the mutation site abolished the siRNA activity.
Our data indicate that siRNAs can be designed to discriminate between the wild type and mutant alleles of genes that differ by just a single nucleotide.
American Psychological Association (APA)
Sierant, Malgorzata& Paduszynska, Alina& Kazmierczak-Baranska, Julia& Nacmias, Benedetta& Sorbi, Sandro& Bagnoli, Silvia…[et al.]. 2011. Specific Silencing of L392V PSEN1 Mutant Allele by RNA Interference. International Journal of Alzheimer's Disease،Vol. 2011, no. 2011, pp.1-14.
https://search.emarefa.net/detail/BIM-499798
Modern Language Association (MLA)
Sierant, Malgorzata…[et al.]. Specific Silencing of L392V PSEN1 Mutant Allele by RNA Interference. International Journal of Alzheimer's Disease No. 2011 (2011), pp.1-14.
https://search.emarefa.net/detail/BIM-499798
American Medical Association (AMA)
Sierant, Malgorzata& Paduszynska, Alina& Kazmierczak-Baranska, Julia& Nacmias, Benedetta& Sorbi, Sandro& Bagnoli, Silvia…[et al.]. Specific Silencing of L392V PSEN1 Mutant Allele by RNA Interference. International Journal of Alzheimer's Disease. 2011. Vol. 2011, no. 2011, pp.1-14.
https://search.emarefa.net/detail/BIM-499798
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-499798