Determination of Hesperetin in Pericarpium Citri Reticulatae and Human Serum Using Flow Injection Chemiluminescence
Joint Authors
Li, Xin
Liu, Jiangman
Tan, Xijuan
Song, Zhenghua
Wang, Jiajia
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-06-24
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
A sensitive chemiluminescence (CL) method for the determination of hesperetin at nanogram levels was first presented.
It was based on the inhibitory effect of hesperetin on luminol-dissolved oxygen CL reaction in a flow injection system.
The decrements of CL intensity were logarithm over the concentrations of hesperetin in a range of 0.5 to 1000 ng mL−1, with a detection limit of 0.2 ng mL−1 (3σ) and the relative standard deviations lower than 2.1%.
At a flow rate of 2.0 mL min−1, the whole determining performance including sampling and washing could be accomplished in 30 s, giving a sampling efficiency of 120 h−1.
The proposed method was applied successfully to the direct hesperetin determination in human serum with recoveries from 97.0 to 106.6%, and indirect hesperidin quantification in 2.5 g pericarpium citri reticulatae showing that the content is 8.1±0.2%.
The possible CL mechanism of luminol-dissolved oxygen-hesperetin reaction was also discussed in detail.
American Psychological Association (APA)
Wang, Jiajia& Tan, Xijuan& Li, Xin& Liu, Jiangman& Song, Zhenghua. 2012. Determination of Hesperetin in Pericarpium Citri Reticulatae and Human Serum Using Flow Injection Chemiluminescence. ISRN Analytical Chemistry،Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-499803
Modern Language Association (MLA)
Wang, Jiajia…[et al.]. Determination of Hesperetin in Pericarpium Citri Reticulatae and Human Serum Using Flow Injection Chemiluminescence. ISRN Analytical Chemistry No. 2012 (2012), pp.1-5.
https://search.emarefa.net/detail/BIM-499803
American Medical Association (AMA)
Wang, Jiajia& Tan, Xijuan& Li, Xin& Liu, Jiangman& Song, Zhenghua. Determination of Hesperetin in Pericarpium Citri Reticulatae and Human Serum Using Flow Injection Chemiluminescence. ISRN Analytical Chemistry. 2012. Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-499803
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-499803