A Pilot Study Comparing HPV-Positive and HPV-Negative Head and Neck Squamous Cell Carcinomas by Whole Exome Sequencing
Joint Authors
Lambin, Philippe
Dowthwaite, Samuel A.
Starmans, Maud H. W.
Basmaji, John
Mymryk, Joe S.
Szeto, Christopher C. T.
Xu, Wei
Barrett, John W.
Nichols, Anthony C.
Franklin, Jason H.
Wehrli, Bret
Yoo, John
Boutros, Paul C.
Todorovic, Biljana
Fung, Kevin
Koropatnick, James
Chan-Seng-Yue, Michelle
Palma, David A.
Kwan, Keith
Dhaliwal, Sandeep
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-12-12
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Background.
Next-generation sequencing of cancers has identified important therapeutic targets and biomarkers.
The goal of this pilot study was to compare the genetic changes in a human papillomavirus- (HPV-)positive and an HPV-negative head and neck tumor.
Methods.
DNA was extracted from the blood and primary tumor of a patient with an HPV-positive tonsillar cancer and those of a patient with an HPV-negative oral tongue tumor.
Exome enrichment was performed using the Agilent SureSelect All Exon Kit, followed by sequencing on the ABI SOLiD platform.
Results.
Exome sequencing revealed slightly more mutations in the HPV-negative tumor (73) in contrast to the HPV-positive tumor (58).
Multiple mutations were noted in zinc finger genes (ZNF3, 10, 229, 470, 543, 616, 664, 638, 716, and 799) and mucin genes (MUC4, 6, 12, and 16).
Mutations were noted in MUC12 in both tumors.
Conclusions.
HPV-positive HNSCC is distinct from HPV-negative disease in terms of evidence of viral infection, p16 status, and frequency of mutations.
Next-generation sequencing has the potential to identify novel therapeutic targets and biomarkers in HNSCC.
American Psychological Association (APA)
Nichols, Anthony C.& Chan-Seng-Yue, Michelle& Yoo, John& Xu, Wei& Dhaliwal, Sandeep& Basmaji, John…[et al.]. 2012. A Pilot Study Comparing HPV-Positive and HPV-Negative Head and Neck Squamous Cell Carcinomas by Whole Exome Sequencing. ISRN Oncology،Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-499813
Modern Language Association (MLA)
Nichols, Anthony C.…[et al.]. A Pilot Study Comparing HPV-Positive and HPV-Negative Head and Neck Squamous Cell Carcinomas by Whole Exome Sequencing. ISRN Oncology No. 2012 (2012), pp.1-9.
https://search.emarefa.net/detail/BIM-499813
American Medical Association (AMA)
Nichols, Anthony C.& Chan-Seng-Yue, Michelle& Yoo, John& Xu, Wei& Dhaliwal, Sandeep& Basmaji, John…[et al.]. A Pilot Study Comparing HPV-Positive and HPV-Negative Head and Neck Squamous Cell Carcinomas by Whole Exome Sequencing. ISRN Oncology. 2012. Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-499813
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-499813