Uncoupling Protein 2 Regulates Palmitic Acid-Induced Hepatoma Cell Autophagy
Joint Authors
Lou, Jiaxin
Wang, Xuejiang
Jiang, Ying
Wang, Yunjiao
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-08-04
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Mitochondrial uncoupling protein 2 (UCP2) is suggested to have a role in the development of nonalcoholic steatohepatitis (NASH).
However, the mechanism remains unclear.
Autophagy is an important mediator of many pathological responses.
This study aims to investigate the relationship between UCP2 and hepatoma cells autophagy in palmitic acid- (PA-) induced lipotoxicity.
H4IIE cells were treated with palmitic acid (PA), and cell autophagy and apoptosis were examined.
UCP2 expression, in association with LC3-II and caspase-3, which are indicators of cell autophagy and apoptosis, respectively,was measured.
Results demonstrated that UCP2 was associated with autophagy during PA-induced hepatic carcinoma cells injury.
Tests on reactive oxygen species (ROS) showed that UCP2 overexpression strongly decreases PA-induced ROS production and apoptosis.
Conversely, UCP2 inhibition by genipin or UCP2 mRNA silencing enhances PA-induced ROS production and apoptosis.
Autophagy partially participates in this progress.
Moreover, UCP2 was associated with ATP synthesis during PA-induced autophagy.
In conclusion, increasing UCP2 expression in hepatoma cells may contribute to cell autophagy and antiapoptotic as result of fatty acid injury.
Our results may bring new insights for potential NASH therapies.
American Psychological Association (APA)
Lou, Jiaxin& Wang, Yunjiao& Wang, Xuejiang& Jiang, Ying. 2014. Uncoupling Protein 2 Regulates Palmitic Acid-Induced Hepatoma Cell Autophagy. BioMed Research International،Vol. 2014, no. 2014, pp.1-14.
https://search.emarefa.net/detail/BIM-499905
Modern Language Association (MLA)
Lou, Jiaxin…[et al.]. Uncoupling Protein 2 Regulates Palmitic Acid-Induced Hepatoma Cell Autophagy. BioMed Research International No. 2014 (2014), pp.1-14.
https://search.emarefa.net/detail/BIM-499905
American Medical Association (AMA)
Lou, Jiaxin& Wang, Yunjiao& Wang, Xuejiang& Jiang, Ying. Uncoupling Protein 2 Regulates Palmitic Acid-Induced Hepatoma Cell Autophagy. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-14.
https://search.emarefa.net/detail/BIM-499905
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-499905