Evaluation of Cytotoxic and Antimicrobial Effects of Two Bt Cry Proteins on a GMO Safety Perspective
Joint Authors
Vasconcelos, Ilka Maria
Grossi de Sá, Maria Fátima
Pessoa, Claudia
Silva, Luciano Paulino
Viana, Martônio Ponte
Pessoa, Igor Parra
Farias, Davi Felipe
de Oliveira, Gustavo Ramos
Carvalho, Ana Fontenele Urano
Beneventi, Magda Aparecida
Soares, Bruno Marques
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-23
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Studies have contested the innocuousness of Bacillus thuringiensis (Bt) Cry proteins to mammalian cells as well as to mammals microbiota.
Thus, this study aimed to evaluate the cytotoxic and antimicrobial effects of two Cry proteins, Cry8Ka5 (a novel mutant protein) and Cry1Ac (a widely distributed protein in GM crops).
Evaluation of cyto- and genotoxicity in human lymphocytes was performed as well as hemolytic activity coupled with cellular membrane topography analysis in mammal erythrocytes.
Effects of Cry8Ka5 and Cry1Ac upon Artemia sp.
nauplii and upon bacteria and yeast growth were assessed.
The toxins caused no significant effects on the viability (IC50>1,000 µg/mL) or to the cellular DNA integrity of lymphocytes (no effects at 1,000 µg/mL).
The Cry8Ka5 and Cry1Ac proteins did not cause severe damage to erythrocytes, neither with hemolysis (IC50>1,000 µg/mL) nor with alterations in the membrane.
Likewise, the Cry8Ka5 and Cry1Ac proteins presented high LC50 (755.11 and >1,000 µg/mL, resp.) on the brine shrimp lethality assay and showed no growth inhibition of the microorganisms tested (MIC>1,000 µg/mL).
This study contributed with valuable information on the effects of Cry8Ka5 and Cry1Ac proteins on nontarget organisms, which reinforce their potential for safe biotechnological applications.
American Psychological Association (APA)
Farias, Davi Felipe& Viana, Martônio Ponte& de Oliveira, Gustavo Ramos& Beneventi, Magda Aparecida& Soares, Bruno Marques& Pessoa, Claudia…[et al.]. 2014. Evaluation of Cytotoxic and Antimicrobial Effects of Two Bt Cry Proteins on a GMO Safety Perspective. BioMed Research International،Vol. 2014, no. 2014, pp.1-14.
https://search.emarefa.net/detail/BIM-499915
Modern Language Association (MLA)
Farias, Davi Felipe…[et al.]. Evaluation of Cytotoxic and Antimicrobial Effects of Two Bt Cry Proteins on a GMO Safety Perspective. BioMed Research International No. 2014 (2014), pp.1-14.
https://search.emarefa.net/detail/BIM-499915
American Medical Association (AMA)
Farias, Davi Felipe& Viana, Martônio Ponte& de Oliveira, Gustavo Ramos& Beneventi, Magda Aparecida& Soares, Bruno Marques& Pessoa, Claudia…[et al.]. Evaluation of Cytotoxic and Antimicrobial Effects of Two Bt Cry Proteins on a GMO Safety Perspective. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-14.
https://search.emarefa.net/detail/BIM-499915
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-499915