The Application of Three-Dimensional Collagen-Scaffolds Seeded with Myoblasts to Repair Skeletal Muscle Defects
Joint Authors
Holden, Kyle
Zhu, Jinhong
Ma, Jianqun
Pan, Haiying
Li, Yong
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-12-12
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Three-dimensional (3D) engineered tissue constructs are a novel and promising approach to tissue repair and regeneration.
3D tissue constructs have the ability to restore form and function to damaged soft tissue unlike previous methods, such as plastic surgery, which are able to restore only form, leaving the function of the soft tissue often compromised.
In this study, we seeded murine myoblasts (C2C12) into a collagen composite scaffold and cultured the scaffold in a roller bottle cell culture system in order to create a 3D tissue graft in vitro.
The 3D graft created in vitro was then utilized to investigate muscle tissue repair in vivo.
The 3D muscle grafts were implanted into defect sites created in the skeletal muscles in mice.
We detected that the scaffolds degraded slowly over time, and muscle healing was improved which was shown by an increased quantity of innervated and vascularized regenerated muscle fibers.
Our results suggest that the collagen composite scaffold seeded with myoblasts can create a 3D muscle graft in vitro that can be employed for defect muscle tissue repair in vivo.
American Psychological Association (APA)
Ma, Jianqun& Holden, Kyle& Zhu, Jinhong& Pan, Haiying& Li, Yong. 2011. The Application of Three-Dimensional Collagen-Scaffolds Seeded with Myoblasts to Repair Skeletal Muscle Defects. Journal of Biomedicine and Biotechnology،Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-499992
Modern Language Association (MLA)
Ma, Jianqun…[et al.]. The Application of Three-Dimensional Collagen-Scaffolds Seeded with Myoblasts to Repair Skeletal Muscle Defects. Journal of Biomedicine and Biotechnology No. 2011 (2011), pp.1-9.
https://search.emarefa.net/detail/BIM-499992
American Medical Association (AMA)
Ma, Jianqun& Holden, Kyle& Zhu, Jinhong& Pan, Haiying& Li, Yong. The Application of Three-Dimensional Collagen-Scaffolds Seeded with Myoblasts to Repair Skeletal Muscle Defects. Journal of Biomedicine and Biotechnology. 2011. Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-499992
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-499992