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Fibroblast-Like Synoviocytes Induce Calcium Mineral Formation and Deposition
Joint Authors
Norton, Harry James
Hanley, Edward N.
Mauerhan, David R.
Zinchenko, Natalia
Gruber, Helen E.
Sun, Yubo
Franklin, Atiya M.
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-20
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Calcium crystals are present in the synovial fluid of 65%–100% patients with osteoarthritis (OA) and 20%–39% patients with rheumatoid arthritis (RA).
This study sought to investigate the role of fibroblast-like synoviocytes (FLSs) in calcium mineral formation.
We found that numerous genes classified in the biomineral formation process, including bone gamma-carboxyglutamate (gla) protein/osteocalcin, runt-related transcription factor 2, ankylosis progressive homolog, and parathyroid hormone-like hormone, were differentially expressed in the OA and RA FLSs.
Calcium deposits were detected in FLSs cultured in regular medium in the presence of ATP and FLSs cultured in chondrogenesis medium in the absence of ATP.
More calcium minerals were deposited in the cultures of OA FLSs than in the cultures of RA FLSs.
Examination of the micromass stained with nonaqueous alcoholic eosin indicated the presence of birefringent crystals.
Phosphocitrate inhibited the OA FLSs-mediated calcium mineral deposition.
These findings together suggest that OA FLSs are not passive bystanders but are active players in the pathological calcification process occurring in OA and that potential calcification stimuli for OA FLSs-mediated calcium deposition include ATP and certain unidentified differentiation-inducing factor(s).
The OA FLSs-mediated pathological calcification process is a valid target for the development of disease-modifying drug for OA therapy.
American Psychological Association (APA)
Sun, Yubo& Mauerhan, David R.& Franklin, Atiya M.& Zinchenko, Natalia& Norton, Harry James& Hanley, Edward N.…[et al.]. 2014. Fibroblast-Like Synoviocytes Induce Calcium Mineral Formation and Deposition. Arthritis،Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-500047
Modern Language Association (MLA)
Sun, Yubo…[et al.]. Fibroblast-Like Synoviocytes Induce Calcium Mineral Formation and Deposition. Arthritis No. 2014 (2014), pp.1-12.
https://search.emarefa.net/detail/BIM-500047
American Medical Association (AMA)
Sun, Yubo& Mauerhan, David R.& Franklin, Atiya M.& Zinchenko, Natalia& Norton, Harry James& Hanley, Edward N.…[et al.]. Fibroblast-Like Synoviocytes Induce Calcium Mineral Formation and Deposition. Arthritis. 2014. Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-500047
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-500047