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Histochemical Expression of Mast Cell Chymase in Chronic Periodontitis and Cyclosporine-Induced Gingival Overgrowth
Joint Authors
Senthilkumar, Kamatchiammal
Periasamy, Soundararajan
Subramani, Tamilselvan
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-04-10
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Mast cell (MC) mediators play a vital role in fibrosis.
The purpose of this study was to investigate the MCs and their enzyme chymase in gingival tissues showing drug-induced gingival overgrowth (DIGO) and also to evaluate the correlation of MC counting and expression with the chronic periodontitis.
In this study, 30 samples, including cyclosporine-induced gingival overgrowth, chronic periodontitis (10 for each), and ten normal gingival tissues, were collected.
We analyzed the histochemical expression of MC chymase in all the collected tissues.
In addition, the number of MCs was counted for each deparaffinized section stained with toluidine blue.
Furthermore, total RNA was extracted from tissue samples, and RT-PCR was performed for MC chymase.
The numbers of MCs were found to be increased in relative lesions compared to normal gingival tissues (P<0.05).
Moreover, chymase-containing MCs in DIGO tissues showed striking differences from those of control subjects and chronic periodontitis (P<0.01).
The RT-PCR analysis further revealed that MC chymase mRNA increased significantly in DIGO tissues.
In conclusion, although the MCs were less numerous in numbers, the cells exhibited significant expression of chymase enzyme suggesting the involvement of MCs in DIGO.
American Psychological Association (APA)
Subramani, Tamilselvan& Senthilkumar, Kamatchiammal& Periasamy, Soundararajan. 2013. Histochemical Expression of Mast Cell Chymase in Chronic Periodontitis and Cyclosporine-Induced Gingival Overgrowth. Journal of Histology،Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-500060
Modern Language Association (MLA)
Subramani, Tamilselvan…[et al.]. Histochemical Expression of Mast Cell Chymase in Chronic Periodontitis and Cyclosporine-Induced Gingival Overgrowth. Journal of Histology No. 2013 (2013), pp.1-5.
https://search.emarefa.net/detail/BIM-500060
American Medical Association (AMA)
Subramani, Tamilselvan& Senthilkumar, Kamatchiammal& Periasamy, Soundararajan. Histochemical Expression of Mast Cell Chymase in Chronic Periodontitis and Cyclosporine-Induced Gingival Overgrowth. Journal of Histology. 2013. Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-500060
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-500060