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Induction of Interleukin-8 from Nasal Epithelial Cells during Bacterial Infection : The Role of IL-8 for Neutrophil Recruitment in Chronic Rhinosinusitis
Joint Authors
Yoon, Bit-Na
Roh, Hwan-Jung
Lee, Hyun-Sun
Cho, Kyu-Sup
Choi, Nan-Geum
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-06-20
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Objectives.
The aim of this study was to elucidate the role of IL-8 for neutrophil recruitment in nonallergic CRS patients.
Methods.
After coculture of Streptococcus pneumoniae (SP) with the mucosal epithelial cells (MECs) from non-CRS patients, at three different SP/MEC (1/1, 10/1, 100/1) ratios, the expression of IL-8 mRNA and the concentration of IL-8 were measured by RT-PCR and ELISA.
The expression of CD11b/CD18 on neutrophils and E-selectin/ICAM-1 on endothelial cells and the adherence between neutrophils and human umbilical vascular endothelial cells (HUVECs) were determined by flow cytometric analysis, ELISA, and RIA, respectively.
Results.
IL-8 concentration and IL-8 mRNA expression continued to increase from 3 hours after incubation in SP number-dependent manner.
The expression of CD11b/CD18 on neutrophils and E-selectin/ICAM-1 on HUVECs, and the adherence between neutrophils and HUVECs were significantly increased in 10 SP/MEC-CM, and the increments were significantly blocked by anti-IL-8 antibody.
Conclusion.
MEC and IL-8 are major factors for neutrophil recruitment in nonallergic CRS.
American Psychological Association (APA)
Yoon, Bit-Na& Choi, Nan-Geum& Lee, Hyun-Sun& Cho, Kyu-Sup& Roh, Hwan-Jung. 2010. Induction of Interleukin-8 from Nasal Epithelial Cells during Bacterial Infection : The Role of IL-8 for Neutrophil Recruitment in Chronic Rhinosinusitis. Mediators of Inflammation،Vol. 2010, no. 2010, pp.1-7.
https://search.emarefa.net/detail/BIM-500127
Modern Language Association (MLA)
Yoon, Bit-Na…[et al.]. Induction of Interleukin-8 from Nasal Epithelial Cells during Bacterial Infection : The Role of IL-8 for Neutrophil Recruitment in Chronic Rhinosinusitis. Mediators of Inflammation No. 2010 (2010), pp.1-7.
https://search.emarefa.net/detail/BIM-500127
American Medical Association (AMA)
Yoon, Bit-Na& Choi, Nan-Geum& Lee, Hyun-Sun& Cho, Kyu-Sup& Roh, Hwan-Jung. Induction of Interleukin-8 from Nasal Epithelial Cells during Bacterial Infection : The Role of IL-8 for Neutrophil Recruitment in Chronic Rhinosinusitis. Mediators of Inflammation. 2010. Vol. 2010, no. 2010, pp.1-7.
https://search.emarefa.net/detail/BIM-500127
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-500127