A Novel Method for Real-Time, Continuous, Fluorescence-Based Analysis of Anti-DNA Abzyme Activity in Systemic Lupus
Joint Authors
Hartmann, James X.
Cavallo, Michelle F.
Kats, Anna M.
Pavlovic, Mirjana
Chen, Ran
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-12-05
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Systemic Lupus Erythematosus (SLE) is an autoimmune disease characterized by the production of antibodies against a variety of self-antigens including nucleic acids.
These antibodies are cytotoxic, catalytic (hydrolyzing DNA, RNA, and protein), and nephritogenic.
Current methods for investigating catalytic activities of natural abzymes produced by individuals suffering from autoimmunity are mostly discontinuous and often employ hazardous reagents.
Here we demonstrate the utility of dual-labeled, fluorogenic DNA hydrolysis probes in highly specific, sensitive, continuous, fluorescence-based measurement of DNA hydrolytic activity of anti-ssDNA abzymes purified from the serum of patients suffering from SLE.
An assay for the presence and levels of antibodies exhibiting hydrolytic activity could facilitate disease diagnosis, prediction of flares, monitoring of disease state, and response to therapy.
The assay may allow indirect identification of additional targets of anti-DNA antibodies and the discovery of molecules that inhibit their activity.
Combined, these approaches may provide new insights into molecular mechanisms of lupus pathogenesis.
American Psychological Association (APA)
Cavallo, Michelle F.& Kats, Anna M.& Chen, Ran& Hartmann, James X.& Pavlovic, Mirjana. 2012. A Novel Method for Real-Time, Continuous, Fluorescence-Based Analysis of Anti-DNA Abzyme Activity in Systemic Lupus. Autoimmune Diseases،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-500164
Modern Language Association (MLA)
Cavallo, Michelle F.…[et al.]. A Novel Method for Real-Time, Continuous, Fluorescence-Based Analysis of Anti-DNA Abzyme Activity in Systemic Lupus. Autoimmune Diseases No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-500164
American Medical Association (AMA)
Cavallo, Michelle F.& Kats, Anna M.& Chen, Ran& Hartmann, James X.& Pavlovic, Mirjana. A Novel Method for Real-Time, Continuous, Fluorescence-Based Analysis of Anti-DNA Abzyme Activity in Systemic Lupus. Autoimmune Diseases. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-500164
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-500164