Anti-leishmanial polyclonal antibodies to assess the performance characteristics of leishmanial antigen detection elisa
Joint Authors
Azazy, Ahmad A.
al-Shaybani, Latifa A.
Muhammad, Atif al-Sayyid
Source
Journal of the Egyptian Society of Parasitology
Issue
Vol. 38, Issue 3 (31 Dec. 2008), pp.1027-1036, 10 p.
Publisher
The Egyptian Society of Parasitology
Publication Date
2008-12-31
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Topics
Abstract EN
The polyclonal antibodies raised in rabbits against amastigote antigen extract were purified and fractionated, and IgG class antibodies and from the same antibodies, a peroxidase conjugate (labeled antibodies) reagent were prepared.
The antibodies and the labeled antibodies were analyzed for efficacy of the homologous extracted antigens by capture ELISA.
The titration curves of the anti-amastigote IgG antibody against extracted antigens showed that both free antibody and corresponding labeled antibody reacted with the original amastigote antigens.
Further analysis involved the interaction between the antibody and two leishmanial stages ; mammalian amastigote & infective promasitgote by immunoflourescene technique.
The strong interaction was not only with surface antigenic components of the stages but also with their internal components.
Capture-ELISA system was done to detect specific leishmanial antigens in urine and sera from visceral leishmaniasis patients (VL).
Most of the urine samples were positive (90 % sensitivity) for leishmanial antigens without cross-reactivity (100 % specificity) with any other tested samples from heterologous parasitic infections.
But, only 61 % sensitivity and 53 % specificity were obtained when the capture ELISA was done to detect the specific leishmanail antigens in sera from VL.
American Psychological Association (APA)
Azazy, Ahmad A.& al-Shaybani, Latifa A.& Muhammad, Atif al-Sayyid. 2008. Anti-leishmanial polyclonal antibodies to assess the performance characteristics of leishmanial antigen detection elisa. Journal of the Egyptian Society of Parasitology،Vol. 38, no. 3, pp.1027-1036.
https://search.emarefa.net/detail/BIM-50020
Modern Language Association (MLA)
Azazy, Ahmad A.…[et al.]. Anti-leishmanial polyclonal antibodies to assess the performance characteristics of leishmanial antigen detection elisa. Journal of the Egyptian Society of Parasitology Vol. 38, no. 3 (Dec. 2008), pp.1027-1036.
https://search.emarefa.net/detail/BIM-50020
American Medical Association (AMA)
Azazy, Ahmad A.& al-Shaybani, Latifa A.& Muhammad, Atif al-Sayyid. Anti-leishmanial polyclonal antibodies to assess the performance characteristics of leishmanial antigen detection elisa. Journal of the Egyptian Society of Parasitology. 2008. Vol. 38, no. 3, pp.1027-1036.
https://search.emarefa.net/detail/BIM-50020
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 1033-1036
Record ID
BIM-50020