Analysis of Alphalactalbumin and Betalactoglobulin from the Rehydration of Bovine Colostrum Powder Using Cloud Point Extraction and Mass Spectrometry
Joint Authors
Zou, Mingqiang
Li, Jinfeng
Qi, Xiaohua
Zhang, Fan
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-4, 4 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-03-11
Country of Publication
Egypt
No. of Pages
4
Main Subjects
Abstract EN
Alphalactalbumin (α-La) and betalactoglobulin (β-Lg) in the rehydration of bovine colostrum powder were successfully separated by cloud point extraction using a nonionic surfactant Triton X-114.
The effects of different factors, including the surfactant concentration, sample volume, electrolyte, and pH were discussed.
The optimized conditions for cloud point extraction of alphalactalbumin (α-La) and betalactoglobulin (β-Lg) can be concluded that the best surfactant is 1% (w/v) Triton X-114, 200 μL of sample volume, 150 mmol/L NaCl, and 6% (w/v) sucrose.
After cloud point extraction, the capillary electrophoresis is used to check the efficiency of the extraction procedure.
The results had been effectively confirmed by the characterization with matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS).
American Psychological Association (APA)
Zhang, Fan& Qi, Xiaohua& Zou, Mingqiang& Li, Jinfeng. 2012. Analysis of Alphalactalbumin and Betalactoglobulin from the Rehydration of Bovine Colostrum Powder Using Cloud Point Extraction and Mass Spectrometry. Journal of Analytical Methods in Chemistry،Vol. 2012, no. 2012, pp.1-4.
https://search.emarefa.net/detail/BIM-500380
Modern Language Association (MLA)
Zhang, Fan…[et al.]. Analysis of Alphalactalbumin and Betalactoglobulin from the Rehydration of Bovine Colostrum Powder Using Cloud Point Extraction and Mass Spectrometry. Journal of Analytical Methods in Chemistry No. 2012 (2012), pp.1-4.
https://search.emarefa.net/detail/BIM-500380
American Medical Association (AMA)
Zhang, Fan& Qi, Xiaohua& Zou, Mingqiang& Li, Jinfeng. Analysis of Alphalactalbumin and Betalactoglobulin from the Rehydration of Bovine Colostrum Powder Using Cloud Point Extraction and Mass Spectrometry. Journal of Analytical Methods in Chemistry. 2012. Vol. 2012, no. 2012, pp.1-4.
https://search.emarefa.net/detail/BIM-500380
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-500380