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Alternative Splicing of a Novel Inducible Exon Diversifies the CASK Guanylate Kinase Domain
Joint Authors
Han, Joonhee
Yeo, Gene
An, Ping
Scoulos-Hanson, Maritsa
Dembowski, Jill A.
Fu, Xiang-Dong
Grabowski, Paula J.
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-09-12
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
Alternative pre-mRNA splicing has a major impact on cellular functions and development with the potential to fine-tune cellular localization, posttranslational modification, interaction properties, and expression levels of cognate proteins.
The plasticity of regulation sets the stage for cells to adjust the relative levels of spliced mRNA isoforms in response to stress or stimulation.
As part of an exon profiling analysis of mouse cortical neurons stimulated with high KCl to induce membrane depolarization, we detected a previously unrecognized exon (E24a) of the CASK gene, which encodes for a conserved peptide insertion in the guanylate kinase interaction domain.
Comparative sequence analysis shows that E24a appeared selectively in mammalian CASK genes as part of a >3,000 base pair intron insertion.
We demonstrate that a combination of a naturally defective 5′ splice site and negative regulation by several splicing factors, including SC35 (SRSF2) and ASF/SF2 (SRSF1), drives E24a skipping in most cell types.
However, this negative regulation is countered with an observed increase in E24a inclusion after neuronal stimulation and NMDA receptor signaling.
Taken together, E24a is typically a skipped exon, which awakens during neuronal stimulation with the potential to diversify the protein interaction properties of the CASK polypeptide.
American Psychological Association (APA)
Dembowski, Jill A.& An, Ping& Scoulos-Hanson, Maritsa& Yeo, Gene& Han, Joonhee& Fu, Xiang-Dong…[et al.]. 2012. Alternative Splicing of a Novel Inducible Exon Diversifies the CASK Guanylate Kinase Domain. Journal of Nucleic Acids،Vol. 2012, no. 2012, pp.1-15.
https://search.emarefa.net/detail/BIM-500387
Modern Language Association (MLA)
Dembowski, Jill A.…[et al.]. Alternative Splicing of a Novel Inducible Exon Diversifies the CASK Guanylate Kinase Domain. Journal of Nucleic Acids No. 2012 (2012), pp.1-15.
https://search.emarefa.net/detail/BIM-500387
American Medical Association (AMA)
Dembowski, Jill A.& An, Ping& Scoulos-Hanson, Maritsa& Yeo, Gene& Han, Joonhee& Fu, Xiang-Dong…[et al.]. Alternative Splicing of a Novel Inducible Exon Diversifies the CASK Guanylate Kinase Domain. Journal of Nucleic Acids. 2012. Vol. 2012, no. 2012, pp.1-15.
https://search.emarefa.net/detail/BIM-500387
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-500387